Sustainable development in the bio-treatment of large-scale biomass bulks requires high performance enzymes adapted to extreme conditions. An extracellular keratinolytic extract was obtained from the culture broth of a halotolerant strain of Salicola marasensis. Keratin hydrolyzing activity of the concentrated enzyme extract was observed on a 100 mg of pretreated feather waste...

Extracellular proteins play an essential role in the formation, differentiation, and maintenance of multicellular organisms. Despite that, the systematic identification of genes encoding these proteins has not been possible. We describe here a highly efficient method to isolate genes encoding secreted and membrane-bound proteins by using a single-step selection in yeast. Application of this met...

The predicted extracellular proteins of the bacterium Lactobacillus plantarum were analysed to gain insight into the mechanisms underlying interactions of this bacterium with its environment. Extracellular proteins play important roles in processes ranging from probiotic effects in the gastrointestinal tract to degradation of complex extracellular carbon sources such as those found in plant mat...

one of the main members of the large aggregating proteoglycans (pgs) family is versican which is able to bind to hyaluronate. versican is a chondroitin sulfate proteoglycan and is a key ingredient of the extracellular matrix.  due to its widespread expression in the body, versican is involved in cell adhesion, proliferation and migration. induced expression of versican is often observed in tiss...

background: to develop a new green approach for biosynthesis of silver nanoparticles, myconanotechnology has been represented as a novel field of study in nanotechnology. in this study, we have reported the extracellular synthesis of highly stable silver nanoparticles using three species of dermatophytes: trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. methods: clinical ...

Lysosomes are organelles that support diverse cellular functions such as terminal degradation of macromolecules and nutrient recycling. Additionally, lysosomes can fuse with the plasma membrane, a phenomenon referred to lysosomal exocytosis, release their contents, including hydrolytic enzymes cargo proteins. Recently, neuronal activity has been shown induce exocytosis in dendrites axons. Secre...

The clinical case of cardiac pseudoamyloidosis associated with multiple myeloma Cardiac amyloidosis is an infiltrative cardiomyopathy resulting from extracellular deposition amyloid- a specific protein-polysaccharide complex. This demonstrates unverified amyloid-like heart lesion in the presence unexplained thickening wall undilated left ventricle and manifestations restrictive failure patient ...

The combination of super-resolution microscopy and synthetic fluorescence probes has emerged as a universal tool to monitor dynamic biological events at the nanometer scale. However, limited site-specificity fluorogenicity fluorescent make it still difficult realize long-term imaging. Herein, we introduce aggregation mediated SNAP-tag fluorogenic probe, BGAN-Aze, which can specifically bind var...