objective(s): although low-dose radiotherapy (rt) that involves low collateral damage is more suitable for hepatocellular carcinoma (hcc) than traditional high-dose rt, but to achieve satisfactory therapeutic effect with low-dose rt, it is necessary to sensitize hcc cells to irradiation. this study was aimed to determine whether radiosensitivity of hcc cells can be enhanced using mir-26b by tar...

the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...

alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. in the present study, alkaline phosphatase gene was inserted into the pet15b vector. the recombinant dna was then expressed using iptg as an inducer. the expression of this enzyme was optimized by changing expression conditions...

background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...

to construct of an eukaryotic expression vector encoding herpes simplex virus type 2 (hsv-2) glycoprotein d (gd2), an iranian isolate of hsv-2 was propagated in hela cell line and its dna was extracted and used as template in polymerase chain reactions (pcr), to amplify gd2 gene. primers were designed and the restriction enzyme sites for ecori and xhoi were considered at their 5′ ends respectiv...

Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study was aimed to determine whether radiosensitivity of HCC cells can be enhanced using miR-26b by tar...

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...

background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...

lentiviral vectors are among the promising viral based-vectors in gene therapy applications, but the efficiency of their targeting needs to be improved. (strept)avidin-biotin adaptor system is a novel approach to modify the lentiviral envelope for better targeting properties. herein, we describe utilization of this adaptor system by designing a candidate envelope protein-bearing biotin acceptor...