background the development of an effective subunit vaccine against brucellosis is a research area of intense. but optimization of recombinant proteins production in escherichia coli and content of endotoxins associated with final recombinant proteins are very important. objectives in the present study, expression and purification of brucella melitensis rhsp and rtf were optimized to reduce endo...

the main purpose in this paper, to express strong algorithmic optimization in their ability to detect expression target of sar that use in aircraft, plane and satellites to observe the target on the ground. for this purpose,the sar technology and its applications are introduced. previous algorithms used in this field with poor diagnosis and appropriate speed is low, like means and fuzzy and pso...

In this paper, a new general scalarization technique for solving multiobjective optimization problems is presented. After studying the properties of this formulation, two problems as special cases of this general formula are considered. It is shown that some well-known methods such as the weighted sum method, the -constraint method, the Benson method, the hybrid method and the elastic -constrai...

Introduction MicroRNAs (miRNAs) are small RNAs which function as regulators of posttranscriptional gene expression by binding to their mRNA targets [1]. MiRNAs are involved in crucial regulations of many signaling and metabolic pathways. In difference to other interfering RNAs (RNAi), miRNAs can target many mRNA, thus having an increased impact on regulation of gene expression. These properties...

background: culture media enrichment through the addition of protein hydrolysates is beneficial for achieving higher protein expression. methods: in this study, designing the optimum mixture of four soy and casein-derived hydrolysates was successfully performed by design of experiment and specific productivity increased in all predicted combinations. protein profile of recombinant cho (rcho) ce...

background: mycobacterium tuberculosis is the causative agent of tuberculosis (tb). bacille calmette-guerin (bcg) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult tb vaccine. the aim of this study was to develop a new tuberculosis dna vaccine candidate encoding a recombinant hspx-ppe44-esxv fusion antigen of m. tuberculosis. methods: a fu...

The DNA microarray technology concurrently monitors the expression levels of thousands of genes during significant biological processes and across the related samples. The better understanding of functional genomics is obtained by extracting the patterns hidden in gene expression data. It is handled by clustering which reveals natural structures and identify interesting patterns in the underlyi...

Porcine β-defensin-2 (pBD2) is a cationic antimicrobial peptide that has therapeutic potential. The amount of pBD2 in nature is limited, and the expression of pBD2 in Escherichia coli is low, probably because a different gene codon is used by prokaryotic organisms to that used by eukaryotes. Codon preference optimization is one of the ways to increase heterologous expression of pBD2. To achieve...