the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...

objective(s) staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. antibiotic therapy for vancomycin resistant s. aureus (vrsa) can not promise the eradication of infections. since adhesion is the major route of infections, adhesin based vaccine could suppress s. aureus infections. fibronectin binding protein a (fnbpa) and clumping factor a (clfa) ...

Objective(s):An earlier meta-analysis on gene expression data derived from four microarray, two cDNA library, and one SAGE experiment had identified RGS5 as one of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (TM) cells by all studies. RGS5 encodes regulator of G-protein signaling-5. The TM tissue is the route of aqueous fluid outflow, and is r...

background and objectives: during crisis, hospitals have great responsibility in saving life and protect health of the damaged individuals. fulfilling this responsibility relies on preparedness of hospital staff, particularly the clinicians to face the relevant challenges. given the lack of adequate information on the topic from iran, the present study aimed to explore the technical crisis prep...

Objective(s): It is now supposed that cytokines released during the burn injuries have a great impact on the immunological and pathological responses after the burn. The main objective of this study was to investigate the expression of some pro-inflammatory genes in the wound, spleen and blood neutrophils during the healing process of burn wounds in a murine model. Materials and Methods: The ex...

background: cell therapy of many neurodegenerative diseases using bone marrow stromal cells (bmsc) requires the differentiation of bmsc into neuronal subtype. however, the transdifferentiation of bmsc into gabaergic phenotype requires more investigation. methods: in this study, bmsc of adult female rats were pre-induced into neuroblast-like cells using 1 mm β-mercaptoethanol (βme) and 10 m ret...

The results of toxicity tests can be used to calculate the potentially affected fraction (PAF) of species in an ecosystem at a given pollutant concentration using statistical extrapolation methods. The PAF curve indicates the fraction of species from the original community that may become inhibited at each elevated pollutant concentration and is a measure of the ecotoxicological risk. Pollution...

Perceptions of the legitimacy courts are generally measured by means surveys among population. As is an abstract concept, it has to be captured simple questions. The questions posed in influential use a variety conceptualisations. Instead asking for opinions about legitimacy, one can also examine whether acceptance demonstrated behaviour. This offers potentially more reliable way measure legiti...