The objective of this study was to determine the prevalence of Shigatoxigenic Escherichia coli (STEC) and STEC serogroups O157, O111 and O113 in feces, water and milk sampled in dairy farms in Jaboticabal, SP, Brazil. Feces (n=454), water (n=54) and milk samples (n=30) were collected from 10 herds and assessed for the presence of the virulence genes stx1, stx2 and eae by polymerase chain reacti...

The large outbreak occurred in Germany during Summer 2011 associated to the consumption of sprouts was caused by a VTEC strain which possessed atypical characteristics, and did not fit the current definition of pathogenic VTEC. In fact, the epidemic strain did not possess the eae gene, encoding the adherence factor intimin, but possessed genes that are typical of the Enteroaggregative Escherich...

Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbria...

The yeast Saccharomyces cerevisiae is a widely used experimental system for basic research in cell biology. Numerous researchers use it because of the ease of genetic manipulations. Two different techniques are often used for genetic manipulations. One is the use of vectors such as the integrative, centromeric, and episomal plasmids of the pRS series (1,2) for gene cloning and the second is the...

We used mechanical alloying with a Spex 8000 mixer/mill to synthesize a series of Fe1002xNix alloys from x50 to x549. The Spex mill was modified so that it could also operate at a reduced milling intensity, and we compared the alloys synthesized after long times with the normal and reduced milling intensities. X-ray diffractometry and Mössbauer spectrometry were used to measure the volume fract...