This review summarizes the use of computer assisted liquid chromatographic method development for the analytical characterization of protein biopharmaceuticals. Several modes of chromatography including reversed-phase liquid chromatography (RPLC), ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC) and some perspectives are discussed. For all these chromatographic mo...

Neocarzinostatin, a highly toxic antitumor protein containing an essential nonprotein chromophore, can be isolated and purified from culture filtrates of Streptomyces carcinostaticus. Usually a lengthy procedure of up to 60 h is necessary for the isolation, including several chromatographic steps partly under conditions which favour inactivation of the drug by release of chromophore. We describ...

A T antigen preparation free of trypsin was obtained by application of CNBr-Sepharose linked to pure trypsin. Purification on an ion exchange chromatography column results in an electrophoretically homogeneous preparation of T protein.

Methods for separation of ions by ion-exchange, ion-pair, and zwitterion ion chromatography share at least one common thread--the induced formation of a cation-anion pair in the stationary phase. Selectivity can be defined as the relative ability of sample ions to form such a pair. Examples are given in anion-exchange chromatography to show the effect of variations in the geometry, bulkiness an...