MOTIVATION The importance of fast and affordable DNA sequencing methods for current day life sciences, medicine and biotechnology is hard to overstate. A major player is Ion Torrent, a pyrosequencing-like technology which produces flowgrams--sequences of incorporation values--which are converted into nucleotide sequences by a base-calling algorithm. Because of its exploitation of ubiquitous sem...

A novel protocol for full-length Mycobacterium tuberculosis gene analysis of first- and second-line drug resistance was developed using the Ion Torrent Personal Genome Machine (PGM). Five genes-rpoB (rifampin), katG (isoniazid), pncA (pyrazinamide), gyrA (ofloxacin/fluoroquinolone), and rrs (aminoglycosides)-were amplified and sequenced, and results were compared to those obtained by genotypic ...

Multi-service residential access networks allow residential customers to choose amongst a variety of service offerings, over a range of Core Networks and subject to user requirements such as QoS, mobility, cost and availability. These issues place requirements on authentication for network access, with a need for mutual authentication of the residential gateway (RG) to the local access point (L...

Using C# as a parser, we process about 3.4 million pieces of data over 680 thousand torrents from thepiratebay.org, and create a graphical representation of the data by infographic. Info-graphic presents the information in an easily readable format, and also can be distributed across many webmediums. Based on the representation/analysis of the data, we are able to determine some interesting cha...

As a major component of the water budget of a wetland, evaporation has proven difficult to measure evapotranspiration accurately. This paper developed a scheme to assess actual evapotranspiration (ETa) across a range of land uses in Nansi Lake Wetland, China, using a remote sensing technique and the Surface Energy Balance Algorithm for Land (SEBAL) model. The estimation of pixel-scaled ETa was ...

This study evaluates seven evapotranspiration models and their performance in water balance studies by using lysimeter measurement data at the Mönchengladbach hydrological and meteorological station in Germany. Of the seven evapotranspiration models evaluated, three models calculate actual evapotranspiration directly using the complementary relationship approach, i.e. the CRAE model of Morton, ...

The original and modified versions of the advection-aridity AA model of regional evapotranspiration are tested with data from the Solar and Meteorological Surface Observation Network SAMSON . The resulting long-term mean annual evapotranspiration estimates are validated against water balances of 25 watersheds that are minimally affected by human activity and contain at least one SAMSON station,...

Cryptosporidium is an important enteric pathogen that infects a wide range of humans and animals. Rapid and reliable detection and characterisation methods are essential for understanding the transmission dynamics of the parasite. Sanger sequencing, and high-throughput sequencing (HTS) on an Ion Torrent platform, were compared with each other for their sensitivity and accuracy in detecting and ...