The thermophilic bacteriophage TP84 is dissociated into its head, tail, and released deoxyribonucleic acid (DNA) by chelating agents such as ethylenediaminetetraacetic acid (EDTA) and phosphate. The phage is more sensitive to EDTA than to phosphate, and dialysis against either agent causes more effective dissociation than standing in their presence. The tail possesses a knobbed structure which ...

Submandibular glands from male rats were homogenized in 0.34 M sucrose and 0.5 mM ethylenediaminetetraacetic acid in 10 mM HEPES buffer at a pH of 7.4. The extract was centrifuged and filtered through nuclepore filters to prepare a granule-rich fraction. Electron dense zymogen granules constituted approximately 85% of the particles in this fraction which also contained about a third of the tota...

Abstract Ethylenediaminetetraacetic acid disodium (EDTA) can form stable complexes with many metals. To improve the leaching rate of gold and consumption thiosulfate, EDTA ammonia were combined copper ions to a copper-EDTA-ammonia-thiosulfate system. Electrochemical methods used study effect ion, EDTA, polarization voltage. The results showed that increasing concentrations ions, promoted dissol...

Soft polymer actuators have myriad applications and therefore gained considerable attention in recent years. However, it remains challenging to prepare soft with predefined shapes. Here, a bilayer actuator (re)programmable shape is prepared from microporous anisotropic polypropylene scaffold thin, pH-responsive liquid crystalline network (LCN) layer. The hydrogen bonds between dimerized benzoic...

The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a ...

A method has been developed for the rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum. Hepatocytes were adequately dissociated into single cells when the cultures were first treated with a combination of trypsin and ethylenediaminetetraacetic acid (EDTA) and then with collagenase. However, when the order was reversed, hepatocytes were inade...

This study was carried out to obtain the best technique for preparation of histological sections of bone with minimal artifacts, preserved tissue and high staining affinity. Different bones from adult female rats were gathered and fixed in 10% neutral buffered formalin (10%NBF) then decalcified in 5 different decalcifying agents. Decalcifiers contained strong acids revealed rapid decalcificatio...

A simple two-step procedure for purification of enolase from germinated spores or vegetative cells of Bacillus megaterium is described. The procedure resulted in a 1,200-fold purification with production of homogeneous enzyme in approximately 75% yield; the enzymes from spores and cells seemed identical. The molecular weight of the native enzyme was 335,000, with a subunit molecular weight of 4...