Plasma estradiol concentrations were determined by radioimmunoassay in various endocrine disorders using antiserum to estradiol-17beta succinyl bovine serum albumin. Clinical significance and diagnostic value of plasma estradiol were assessed in hypothalamic-pituitary, adrenal and gonadal disorders. In general, estradiol concentration was correlated well with the degree of sexual maturity and w...

Conditions are described under which prolonged estradiol retention and estrogenic activity are observed in human breast cancer cells in tissue culture. The cells were incubated for three hr with a physiological concentration of [3H]estradiol (3 to 5 nM) and then were washed with 3 successive exchanges of medium 3, 17, and 24 or 48 hr following incubation with [3H]estradiol. The total wash perio...

four crossbred cows with mummified fetus were utilized for the study. the cows were subjected to gynecological examination and based on the findings the cases were diagnosed as mummified fetus. the cows were treated with 2 mg estradiol valerate and 500 µg cloprostenol and were examined every 12 hr after 24 hr of the treatment for cervical dilatation and other signs related to fetal expulsion. t...

objective: foeniculum vulgare seed (f. vulgare) is a herbal plant which is used with phytoestrogene compounds for polycystic ovary syndrome (pcos) treatment. in this research, renoprotective effect of the aqueous extract of foeniculum vulgare (aef) in experimental pcos female rats is studied. materials and methods: forty female rats were randomly divided into five groups. the first group served...

The steroidogenic lesion produced in Leydig cells by gonadotropin stimulation has been attributed to an inhibitory effect of estrogen on 17a-hydroxylase and 17,20-desmolase activity, with impaired conversion of progesterone to androgens. In animals treated with doses of human chorionic gonadotropin (hCG) or gonadotropin-releasing hormone (GnRH) that caused the decrease in desmolase activity, ad...

Systemic estradiol treatment enhances hippocampus-dependent memory in ovariectomized rats. Although these enhancements are traditionally thought to be due to circulating estradiol, recent data suggest these changes are brought on by hippocampus-derived estradiol, the synthesis of which depends on gonadotropin-releasing hormone (GnRH) activity. The goal of the current work is to test the hypothe...

The role of estradiol in mediating leptin's effects on body weight was assessed in ovariectomized (OVX) mice before and after the onset of obesity. Ovariectomy did not alter leptin levels before the onset of obesity, and estradiol adminstration (0.05-17 μg/day for 14 days) did not significantly alter leptin levels if they were corrected for the estradiol-induced reduction in body fat. The conve...

INTRODUCTION The purpose of the study is to compare the effects of genistein, estradiol, estradiol/progesterone combination on the bone mineral density and biomechanical properties of ovariectomized rats' bone. METHODS 50 female adult Sprague-Dawley rats were divided into five groups. Bilaterally ovaeriectomy were performed in all groups except the sham-operated group. Groups were a sham-oper...

BACKGROUND There is a lack of consensus about the safety of estrogen replacement therapy, especially with regard to its impact on a woman's risk for breast cancer. Elevated urinary or serum estrone and estradiol concentrations in postmenopausal women are associated with a moderately elevated risk of breast cancer. METHODS Twenty-four-hour urinary steroid hormone profiles, including the measur...