PREMISE OF THE STUDY The first set of expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized for Speranskia tuberculata (Euphorbiaceae), a traditional medicinal plant endemic to northern China, to explore the effects of recent habitat fragmentation on the genetic diversity and structure of this species. METHODS AND RESULTS In this study, a total of 18...

Genomic resources such as single nucleotide polymorphism (SNPs), insertions and deletions (InDels) and SSRs (simple sequence repeats) are essential for crop improvement and better utilization in genetic breeding. However, the resources for the sacred lotus (Nelumbo nucifera Gaertn.) are still limited. In the present study, to dissect large-scale genomic molecular marker resources for sacred lot...

The bay scallop (Argopecten irradians irradians Lamarck 1819) has become one of the most important aquaculture species in China. Genetic improvement of cultured bay scallop can bene¢t greatly from a better understanding of its genome. In this study, we developed ampli¢ed fragment length polymorphisms (AFLPs) and simple sequence repeat markers from expressed sequence tags (EST-SSRs) for linkage ...

Microsatellites or SSRs (simple sequence repeats) are ubiquitous short tandem duplications occurring in eukaryotic organisms. These sequences are among the best marker technologies applied in plant genetics and breeding. The abundant genomic, BAC, and EST sequences available in databases allow the survey regarding presence and location of SSR loci. Additional information concerning primer seque...

Grasspea (Lathyrus sativus L., 2n = 14) has great agronomic potential because of its ability to survive under extreme conditions, such as drought and flood. However, this legume is less investigated because of its sparse genomic resources and very slow breeding process. In this study, 570 million quality-filtered and trimmed cDNA sequence reads with total length of over 82 billion bp were obtai...

ABBREVIATIONS: AFLP (Amplified Fragment Length Polymorphism); BAC (Bacterial Artificial Chromosome); CAPS (Cleaved Amplified Polymorphic Sequence); CPCSD (California Planting Cotton Seed Distributors); EST (Expressed Sequence Tag); FOV (Fusarium oxysporum f. sp. vasinfectum); FW (Fusarium Wilt); QTL (Quantitative Trait Locus); RAPD (Random Amplified Polymorphic DNA); RIL (Recombinant Inbred Lin...

Abbreviations: ASP: active server page; BLAST: Basic Local Alignment Search Tool; dbEST ID: database for expressed sequence tagsIdentity; EST: expressed sequence tag; GO: Genome ontology; NCBI: National Centre for Biotechnology Information; NSH: no significant homology; RPS-BLAST: Reversed Position Specific BLAST; SSH: subtractive suppressive hybridization; SSR: simple sequence repeats; TIGR: T...