materials and methods: in order to achieve this, recombinant plasmid harboring antisense sequences targeting both of pta and acka was assembled, after transfecting to the cells, its effects on the cell growth and acetate accumulation in the minimal media was assessed and compared with the control, the plasmid without antisense cassette, in presence and absence of iptg in escherichia coli bl21 (...

introduction: the a im of this study is the evaluation of the effect of antimicrobial photodynamic therapy with radachlorin on staphylococcus aureus and escherichia coli. new windows are open in the antimicrobial field so-call photodynamic therapy that incorporates a nonpoisonous photosensitizer (ps) with innocuous special wavelength photons to excite the ps. methods: two strains of bacteria us...

escheric hia coli o157:h7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . detection of the low infectious dosage of bacterium requires a sensitive method. we developed polymerase chain reaction (pcr) assays to detect the gene stx2 irrespective of the bacterial serotype. in this study, the detection limit of the pcr protocol in detecting stx2 ...

b ackground: the prevalence of antibiotic resistance among extended spectrum  β-lactamase  (esbl)  producing  escherichia  coli  and  klebsiella  pneumoniae  has been increased  markedly  in recent  years. the present study was done to know the prevalence  of esbl production among isolates of e. coli and k. pneumoniae and to study the susceptibility pattern of isolates against different antibio...

> introduction: escherichia coli is one of the important pathogens in human with global importance. because of the necessity for identification and the use of novel antibacterial compounds against e. coli , in this present study we focused on the antibacterial effects of synthesized thiazole, imidazole and tetrahydropyridine derivatives on e. coli . methods : for evaluation of antibacterial eff...

the aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofescherichia coli in chicken carcasses by polymerase chain reaction (pcr). in this study 63 strains of e. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of shahrekord. polymerase chain reaction assays were used to detect t...

Escherichia coli strains of serogroup O26 comprise two distinct groups of pathogens, characterized as enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC). Among the several genes related to type III secretion system-secreted effector proteins, espK was found to be highly specific for EHEC O26:H11 and its stx-negative derivative strains isolated in European countries. E. coli O2...

Enterohaemorrhagic E. coli (EHEC) O157:H7 is a primary food-borne bacterial pathogen capable of causing life-threatening human infections which poses a serious challenge to public health worldwide. Intimin, the bacterial outer-membrane protein, plays a key role in the initiating process of EHEC infection. This activity is dependent upon translocation of the intimin receptor (Tir), the intimin b...