High pressure homogenization (HPH) is a non-thermal method, which has been employed to change the activity and stability of biotechnologically relevant enzymes. This work investigated how HPH affects the structural and functional characteristics of a glucose oxidase (GO) from Aspergillus niger. The enzyme was homogenized at 75 and 150 MPa and the effects were evaluated with respect to the enzym...

in this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (znfe2o4@sio2-nh2) is reported. for doing so, at first, the magnetic nanoparticles of znfe2o4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (teos) and 3-aminopropyltriethoxy silane (aptes) were used for modification of znfe2o4 nanoparticles wi...

in the present study, some techniques were used for soybean peroxidase (sbp) purification including: ammonium sulfate fractionation, deae sephadex anion exchange chromatography and concanavalin a sepharose 4b affinity chromatography. molecular weight of purified sbp was estimated about 44 kda by sds-page as a single polypeptide band. the optimal ph and temperature for enzyme activity were found...

Enzymes have been widely studied for their excellent efficiency, selectivity and environmentally benign nature. However, the development of biocatalytic applications is severely limited by lack stability reusability. Nanostructured materials demonstrated as efficient hosts immobilization enzyme due to large surface areas well higher activities long-term stability. Furthermore, nanomaterials wit...

Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme from pitaya peel was optimized in this study. The effect of extraction variables, namely buffer to sample ratio (2:1 to 8:1, X₁), extraction t...

An extracellular xylanase from the thermophilic fungus Melanocarpus albomyces IIS 68 was evaluated for its activity and stability in the presence of polyols and salts at 60 °C, and found to be an effective protecting agent for thermal deactivation of enzyme. Response surface methodology was employed to study the synergistic effects of glycerol and NaCl (thermostabilizers) for xylanase stability...

We studied the immobilization of a recombinant thermostable lipase (Pf2001Δ60) from the hyperthermophilic archaeon Pyrococcus furiosus on supports with different degrees of hydrophobicity: butyl Sepabeads and octadecyl Sepabeads. The enzyme was strongly adsorbed in both supports. When it was adsorbed on these supports, the enzyme showed 140 and 237% hyperactivation, respectively. The assessment...

Enzymes have several excellent catalytic features, and the last few years seen a revolution in biocatalysis, which has grown from using one enzyme to multiple enzymes cascade reactions, where product of reaction is substrate for subsequent one. However, stability remains an issue despite many benefits system. When are exposed harsh process conditions, deactivation occurs, changes activity enzym...

Vitiligo is a depigmentation disorder; Reactive Oxygen Species (ROS) plays a major role in the development of depigmentation. Monoamine oxidase-A(MAO-A) enzyme is one of the reasons in producing ROS by metabolizing norepinephrine which found to be high level in vitiligo patients. Inhibiting MAO-A enzyme prevents further depigmentation. Clinical studies proved that the isoquinoline alkaloids hav...

Chemical modification of α-amylase from locale bacteria isolate Bacillus subtilis ITBCCB148 using glyoxylic acid to increase thermal stability of the enzyme has successfully been done. The results showed that the native and the modified enzyme have similar pH of 6.0 and optimum temperature of 60°C. However, the thermal stability of the modified enzymes was increased 1.8-2.1 times compared to th...