In an ongoing programme to develop characterization strategies relevant to biosensors for in-vivo monitoring, glucose biosensors were fabricated by immobilizing the enzyme glucose oxidase (GOx) on 125 μm diameter Pt cylinder wire electrodes (Pt(C)), using three different methods: before, after or during the amperometric electrosynthesis of poly(ortho-phenylenediamine), PoPD, which also served a...

Background: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial...

In order to address the challenges associated with free lipase in organic solvents, including aggregation, poor stability, and low catalytic efficiency, this study developed two types of poly(o-phenylenediamine) microspheres (solid hollow) as supports for immobilizing lipase. The immobilization process utilized an adsorption method, hollow being identified optimal support a 2:5 enzyme-to-suppor...

The objective of this work is investigation of the adsorption and decomposition of Reactive Blue 19 from industrial wastewaters using laccase enzyme immobilized on activated carbon powder and fiber as adsorbent. Time, pH, temperature, stirring rate, the amount of the adsorbent, dye initial concentration, solution flow rate in the column and column height were studied as key operating parame...

The immobilization of cross-linked tannase onto carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) was achieved via physical adsorption. Glutaraldehyde used to cross-link the enzyme molecules. Spectroscopic and morphological characterizations enzyme-nanotubes composite were carried out, which authenticated successful adsorption event. Enzyme is proven equal to, or even superior ...

6 Visual DSD code for PI controller implementations 13 6.1 Chemical Reaction Network implementation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 6.2 4Domain DNA Strand Displacement implementation . . . . . . . . . . . . . . . . . . . . . . . . . . 16 6.2.1 Initial Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 6.2.2 Reac...

Under aerobic conditions, tyrosinase is inactivated by dopa as a result of suicide inactivation, and, under anaerobic conditions, as a result of irreversible inactivation. However, tyrosine protects the enzyme from being inactivated by dopa under anaerobic conditions. This paper describes how under aerobic conditions the enzyme acting on tyrosine is not directly inactivated but undergoes a proc...