Previous papers in this series have described the crystallization and preliminary characterization of beef plasma monoamine oxidase (1) as well as the fact that cupric copper is one of the prosthetic groups of this enzyme (2). In the present paper, studies related to the second prosthetic groups of this enzyme are presented and the results discussed. A preliminary report has already been publis...

The data provide additional support of the characterization of the biophysical and biochemical properties of the enzyme acetohydroxyacid synthase from the hyperthermophilic bacterium Thermotoga maritima (Eram et al., 2015) [1]. The genes encoding the enzyme subunits have been cloned and expressed in the mesophilic host Escherichia coli. Detailed data include information about the optimization o...

Quercetinase is an enzyme excreted by the mold, Aspergillus flavus when grown on rutin. Characterization of the enzyme involves first isolating and purifying the enzyme from media in which A. flavus has been grown. Improved techniques for this isolation are presented using methods including lectin affinity chromatography and capillary electrophoresis. A pure sample of quercetinase has been obta...

In this article, I reflect on research on two ATPases. The first is F(1)F(0)-ATPase, also known as ATP synthase. It is the terminal enzyme in oxidative phosphorylation and famous as a nanomotor. Early work on mitochondrial enzyme involved purification in large amount, followed by deduction of subunit composition and stoichiometry and determination of molecular sizes of holoenzyme and individual...