to determine the importance of q 10 h 2 as an antioxidant in cancer, we measured the activity of lipoamide dehydrogenase (q 10 h 2 recycling enzyme) in hl-60 and normal lymphocyte. the cultured cells of hl-60 and human normal lymphocytes were assayed in cell lysate of given number of both hl-60 and normal lymphocyte. the activity of lipoamide dehydrogenase and the protein concentration were det...

bacillus sp. guf8, isolated from acidic soil samples of a tea farm was identified as bacillus cereus, based on 16s rdna sequencing and standard bacterial identification methods. following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. consequently, thermostability and kinetic parameters of the purified enzyme wer...

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

the structural requirements for the inhibitor activity of various furan carboxanilide derivatives against succinate dehydrogenase complex (sdc) activity in mitochondria of either wild or mutant strains of ustilago maydis were investigated with the aid of hansch qsar analysis. it has been found that the inhibitor activity against both types of enzymes is best related to the ??? or ??m of the sub...

glucose 6-phosphate dehydrogenase (g6pd) from streptomyces aureofaciens was purified and denatured in 6 m urea. denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...

ceruloplasmin (cp) is a human plasma protein with multiple physiological functions including ferroxidase and oxidase activities. deficiency or inhibition of the enzyme may lead to some abnormalities, including iron deposition in several tissues and causes various pathological conditions in the body. direct interaction of cadmium (cd), a widespread, highly toxic environmental pollutant, with hum...

background and objectives: proline dehydrogenase (prodh; 1.5.99.8) plays an important role in specific determination of plasma proline level in biosensor and diagnostic kits. the goal of this research was to isolate and characterize prodh enzyme from iranian soil microorganisms. materials and methods : screening of l-proline degradative enzymes from soil samples was carried out employing enrich...

Filamentous fungi Trichoderma spp. are efficient hyper-producers of industrially important enzymes. Xylanases are essential hemicellulases having vast ranges of applications in the various industrial sectors. In this study, the protoplast fusion of two T. virens and </span...

Glucose 6-phosphate dehydrogenase (G6PD) from Streptomyces aureofaciens was purified and denatured in 6 M urea. Denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. Dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...