In a biorefinery context it is an advantage to fractionate and extract different wood components in a relatively pure form. However, one major obstacle for efficient extraction of wood polymers (lignin, polysaccharides etc.) is the covalent lignin-polysaccharide networks present in lignified cell walls. Enzymatic catalysis might be a useful tool for a controlled degradation of these networks, t...

background: in algeria, date palm is currently confronted to the bayoud disease. biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species.objectives: callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars.materials and methods: some factors influencing the isolation ...

In recent years, ultrasound (US) has been successfully used in a large number of crucial bioprocesses such as upregulation of enzymatic hydrolysis (biocatalysis). Ultrasound treatment or pretreatment are supposed to activate/accelerate enzyme catalysis enhancing product formation. Such methods might offer high efficiency of enzymatic bioconversion and production of new biologically active pepti...

Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...

L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...

The present work deals with enzymatic hydrolysis of sweet sorghum bagasse to fermentable sugars. The bagasse was treated with phosphoric acid and sodium hydroxide prior to enzymatic hydrolysis by commercial cellulase and β-glucosidase. The phosphoric acid pretreatment was performed at 50°C for 30 min, while 12% NaOH was used for the alkali treatment at 0°C for 3 h. The phosphoric acid pretreatm...

The effectiveness of an enzymatic cocktail, collagenase and trypsin, on the absorptive function of ilealsegment was studied to evaluate the changes within groups after performing ileocystoplasty in an animalmodel. Twenty-one female mixed-bred Persian dogs were randomized into 7 groups. The groups included: 1) negative control group (NCG) in which no ileocystoplasty was performed (n = 3); 2) pos...

Nanocellulose is generally prepared from natural cellulose fermented by higher plants, seaweeds and microorganisms using nano-chemical processes such as acid hydrolysis, mechanical treatment, oxidation enzymatic degradation. This article starts with the definition classification of nanocellulose, focuses on preparation process method nanocellulose.

Enzymatic versus chemical deinking is examined for MOW and photocopy prints. Several enzymatic preparations and two fibre/ink particle separation methods are tested. Deinking was monitored by image analysis and standard pulp and paper characterisation procedures. The effectiveness of the fibre/ink particle separation method depends on the ink particle's size: for smaller particles a washing ste...