to determine the extent and the size of the environment pollution with heavy metals, biologicalmethods, investigating the enzymatic activity of soil can be applied. most frequently, the enzymes investigated in the soils threatened with anthropogenic contamination are phosphatases, dehydrogenases as reacting fastest to the increase in the content of heavy metals in the environment. the aim of th...

essential prerequisite for understanding the underlying chemical processes in biological systems, well before the advent of modern methods for overexpression and affinity tagging 2. In addition to the most basic task of identifying distinct enzymes that catalyze distinct reactions (an endeavor that continues in the genomic era), enzymes were shown to exhibit high specificity, enforce stereochem...

Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37 ?C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46 was obtained in 2%w/v Sodium alginate solution. Investiga...

ABSTRACT. An electrochemical assay of the enzyme alkaline phosphatase (ALP) using ascorbic acid 2-phosphate (AAP) as a new voltammetric substrate has been described in this paper. In the alkaline buffer solution the ALP enzymatic hydrolysis product of AAP was ascorbic acid (AA), which was an electro-active substance and had a sensitive differential pulse voltammetric (DPV) oxidative response on...

The many recent dry-grind plants that convert corn to ethanol are potential sources of substantial amounts of corn oil. This report describes an aqueous enzymatic extraction method to separate oil from dry-fractionated corn germ. The method is an extension of a method previously developed for wet-mill germ. Oil dispersed in lipid bodies throughout the germ was converted to oil droplets suspende...

Silica monoliths with uniform macro-/mesoporous structures (20 μm and 20 nm macro- mesopores diameters, respectively), high porosity (83 %) surface area (370 m2 g−1) were prepared. The grafted amino groups (0.9 mmol NH2 used to immobilize laccase from Trametes versicolor by covalent grafting glutaraldehyde (GLU) (1.0 GLU leading an ABTS activity of U g−1. Immobilization yield was 80 %, based on...

We examine a new approach to reversibly modulate hydrophobic interactions in associative polymers using cyclodextrins (CD) and enzymes that cause scission of the a-1, 4 linkages in cyclodextrins. The associative polymers have a comb-like structure with pendant hydrophobic groups randomly attached to the polymer backbone. The intermolecular interaction between hydrophobic groups forms a transien...