Description: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today truly an essential publication for researchers in all fields ...

Laura Fancellu, MD Giovanni Andrea Deiana, PhD GianPietro Sechi, MD A 41-year-old man with chronic hypertension presented in a transient, global confusional state. The patient had neuropathic pain and kidney disease, the significance not recognized by his doctors. Family history was positive for hypertension and kidney disease. Brain CT, MRI, and magnetic resonance angiography showed abnormalit...

Diacylglycerol kinases (DGKs) are a family of enzymes that catalyze the ATP-dependent phosphorylation of diacylglycerol (DAG) to phosphatidic acid (PtdOH). The recognition of the importance of these enzymes has been increasing ever since it was determined that they played a role in the phosphatidylinositol (PtdIns) cycle and a number of excellent reviews have already been written [(see van Blit...

Ribonuclease A has been the most studied enzyme of the twentieth century. Work on ribonuclease A has endowed enzymology, as well as protein science, with many paradigms. This work continues to provide a framework for understanding protein structure – function relationships in atomic detail. In addition, knowledge gained by basic research is enabling the rapid deployment of ribonuclease A homolo...

* Supported in part by a grant from the Atomic Energy Commission, Contract No. AT (30-1) 1017. t Public Health Research Fellow of the National Cancer Institute. 1 Yanofsky, C., J. Biol. Chem., 223, 171 (1956). 2 Yanofsky, C., and M. Rachmeler, Biochim. et Biophys. Acta, 28, 640 (1958). 3 DeMoss, J. A., M. Imai, and D. M. Bonner, Bact. Proc., 112 (1958). 4Yanofsky, C., Biochem. et Biophys. Acta,...

Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...

Copoazú is a fruit originated from Colombian Amazon belonging to Theobroma genus, which includes species such as cacao raw material for chocolate. Both presented similar processing stages, with different process parameters due the physical qualities of each fruit. Enzymatic, mechanical, and combined methods pulping copoazú seeds their effect on biochemical dynamics fermentation were evaluated. ...