An ink containing tyrosinase catalyzes the tyrosine residues on lysozyme protein to bind it a plasma-treated polyamide-6,6 (PA) fabric. Inkjet printing enables controlled and sequential deposition of two enzymes PA which is necessary for proper binding. The effect different sequences crosslinking stability enzymatic activity presented. bound fabric shows satisfactory antimicrobial activity. pri...

Abstract Sulfoglycolipid, SQAP, is a radiosensitizing agent that makes tumor cells more sensitive to radiation therapy. A previous study revealed SQAP induced the degradation of hypoxia-inducible factor-1α (HIF-1α) and inhibited angiogenesis in hepatoma model mouse. Herein, we examined biological activities against hepatocarcinoma under low oxygen conditions. Cell growth inhibition hypoxic cond...

The inhibition of yeast alcohol dehydrogenasel activity produced by 1, lo-phenanthroline, a zinc-binding reagent, has been studied intensively as an example of the action of a chelating agent on a metalloenzyme. The data (l-3) indicate that OP brings about two distinct types of inactivation of YADH: (a) an instantaneous2 and reversible inhibition and (b) a time-dependent and irreversible inhibi...

materials and methods the expression of hyaluronidase antigenic fragments was optimized using iptg concentration, time of induction, temperature, culture, and absorbance of 0.6-0.8-1 at 600 nm. afterwards, the expressed proteins were purified and the enzymatic activity was assessed by turbid metric method. results data indicated that maximum protein is produced in od = 0.8, 0.5 mm isopropyl β-d...

thermoplastic starch (tps) materials present several advantages to the plastic industry andwhen blended with other materials they can exhibit improved mechanical and moisturesensitivity properties compared to pure tps materials. further investigations on tps: pvablends are of particular interest due to their excellent compatibility and improved properties suchas tensile strength, elongation, to...

enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewat...

Abstract The purpose of the present study is to investigate role hydrogen sulfide (H 2 S), in improving resistance common bean salt stress. Method shows that seeds were soaked water and two concentrations sodium hydrosulfide (50 100 µM) for 8 h. After 25 days from sowing, pots irrigated with NaCl (75 150 mM) until end experiment. Results revealed H S relieved stress by decreasing growth inhibit...

Methylmalonyl-CoA mutase is a key enzyme in intermediary metabolism, and children deficient in enzyme activity have severe metabolic acidosis. We found that nitric oxide (NO) inhibits methylmalonyl-CoA mutase activity in rodent cell extracts. The inhibition of enzyme activity occurred within minutes and was not prevented by thiols, suggesting that enzyme inhibition was not occurring via NO reac...

Product inhibition of cellulolytic enzymes affects the efficiency of the biocatalytic conversion of lignocellulosic biomass to ethanol and other valuable products. New strategies that focus on reactor designs encompassing product removal, notably glucose removal, during enzymatic cellulose conversion are required for alleviation of glucose product inhibition. Supported by numerous calculations ...