escheric hia coli o157:h7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . detection of the low infectious dosage of bacterium requires a sensitive method. we developed polymerase chain reaction (pcr) assays to detect the gene stx2 irrespective of the bacterial serotype. in this study, the detection limit of the pcr protocol in detecting stx2 ...

b ackground: the prevalence of antibiotic resistance among extended spectrum  β-lactamase  (esbl)  producing  escherichia  coli  and  klebsiella  pneumoniae  has been increased  markedly  in recent  years. the present study was done to know the prevalence  of esbl production among isolates of e. coli and k. pneumoniae and to study the susceptibility pattern of isolates against different antibio...

> introduction: escherichia coli is one of the important pathogens in human with global importance. because of the necessity for identification and the use of novel antibacterial compounds against e. coli , in this present study we focused on the antibacterial effects of synthesized thiazole, imidazole and tetrahydropyridine derivatives on e. coli . methods : for evaluation of antibacterial eff...

the aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofescherichia coli in chicken carcasses by polymerase chain reaction (pcr). in this study 63 strains of e. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of shahrekord. polymerase chain reaction assays were used to detect t...

Escherichia coli strains isolated from children with diarrhea were collected from 16 hospitals in different districts in Thailand during 1985 and 1986 and submitted to the National Reference Laboratory. Isolates were identified by serogrouping or as enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC) adhesin factor (EAF) E. coli, or Shiga-like-toxin (S...

Diarrhoeal diseases are one of the most important causes of illness and death all over the world. In Nigeria, the aetiology of diarrhoeagenic bacteria and the virulence of various Escherichia coli pathotypes have not been well-studied because most currently-published data were from the southwestern axis of the country. In total, 520 stool samples were collected from infants, young children, and...

Among strains of Shiga-toxin (Stx) producing Escherichia coli (STEC), seven serogroups (O26, O45, O103, O111, O121, O145, and O157) are associated with severe clinical illness in humans. These strains are also called enterohemorrhagic E. coli (EHEC), and the development of methods for their reliable detection from food has been challenging thus far. PCR detection of major EHEC virulence genes s...

Initial adherence to host cells is the first step of the infection of enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains. The importance of this step in the infection resides in the fact that (1) adherence is the first contact between bacteria and intestinal cells without which the other steps cannot occur and (...