Because water dissolves in silicate melts as at least two species, H O molecules and OH groups, the exsolution 2 Ž . evaporation enthalpy of the H O component from a silicate melt depends on species proportions, which in turn depends on 2 H O pressure. In this short communication, a formal analysis of the exsolution enthalpy of water from a silicate melt is 2 presented, taking into account of t...

Abstract Recently, one of the authors suggested calculating void fraction, an essential element in thermalhydraulics, working with the ‘thermodynamic’ quality instead of the usual ‘flow’ quality. However, the standard heat balance is currently stated as a function of the ‘flow’ quality. Therefore, we should search a new energy balance between the mixture enthalpy, based on ‘thermodynamic’ quali...

Starch and proteins are major components in the wheat endosperm that affect its end product quality. Between the two textural classes of wheat i.e. hard and soft, starch granules are loosely bound with the lipids and proteins in soft wheat due to higher expression of interfering grain softness proteins. It might have impact on starch granules properties. In this work for the first time the phys...

The source of affinity for substrates of human nucleoside diphosphate (NDP) kinases is particularly important in that its knowledge could be used to design more effective antiviral nucleoside drugs (e.g., AZT). We carried out a microcalorimetric study of the binding of enzymes from two organisms to various nucleotides. Isothermal titration calorimetry has been used to characterize the binding i...

Characterization of the thermodynamics of DNA- drug interactions is a very useful part in rational drug design. Isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC) and UV melting experiments have been used to analyze the multivalent (intercalation plus minor groove) binding of the antitumor antibiotic chartreusin to DNA. Using DNA UV melting studies in the presence o...

The binding of AMP to rabbit muscle glycogen phosphorylase a (EC 2.4.1.1.) has been studied by equilibrium dialysis and isothermal microcalorimetry at pH 6.9 over a temperature range of 25 degrees C to 35 degrees C. Thermal titration experiments were carried out in various buffer systems. We have found by these methods that a certain number of protons are released when the protein binds to the ...

The binding of metal ions at the interface of protein complexes presents a unique and poorly understood mechanism of molecular assembly. A remarkable example is the Rad50 zinc hook domain, which is highly conserved and facilitates the Zn2+-mediated homodimerization of Rad50 proteins. Here, we present a detailed analysis of the structural and thermodynamic effects governing the formation and sta...

Reaction microcalorimetry and potentiometry have been used to define the thermodynamics of assembly of Escherichia coli aspartate transcarbamoylase (aspartate carbamoyltransferase, carbamoylphosphate:L-aspartate carbamoyltransferase, EC 2.1.3.2) from its catalytic and regulatory subunits and the linkage between assembly and proton binding. Over the pH range 7-9.5 and the temperature range 15-30...

Analysis of multiple equilibria where the binding enthalpy of the species is the same for all sites and independent of those that are already bonded delivers insight on the evolution of the entropy as the reactions proceed and explains why the equilibrium constants always decrease with increasing number of occupied sites. The validity of the results is independent of the nature of bonding. Cons...