This enterotoxin retained most of its activity after 30 min. at 1OO"C, but was inactivated after 30 min. at 121°C. It will therefore be referred to as the heat-stable enterotoxin or ST. Although ST could occasionally be detected intracellularly in bacteria grown on soft agar cultures, it was mainly extracellular. In some strains of E. coIi its production was controlled by a transmissible plasmi...

OBJECTIVES To investigate the prevalence and toxin production characteristics of non-emetic and emetic Bacillus cereus strains isolated via the laboratory surveillance system in Korea. METHODS A total of 667 B. cereus strains were collected by the Korea National Research Institute of Health laboratory surveillance system from 2012 to 2014. The collected strains were analyzed by geographical r...

A duplex PCR assay was developed for the rapid and specific amplification of the alpha-toxin (phospholipase C, plc) gene and the enterotoxin (cpe) gene from Clostridium perfringens. Two pairs of primers were newly designed for the species identification and also for the differentiation between enterotoxin-positive and enterotoxin-negative C. perfringens strains in a single reaction. The detecti...

BACKGROUND Shigella enterotoxin 1 is a novel enterotoxin elaborated by Shigella flexneri 2a that causes fluid accumulation in rabbit ileal loops and a rise in short circuit current in Ussing chambers. AIMS To gain insights into the mechanism of action of shigella enterotoxin 1. METHODS Supernatants from genetically engineered clones either overexpressing shigella enterotoxin 1 or producing ...

We found that Vibrio cholerae 569B produced much more cholera enterotoxin in the presence of added carbon dioxide than in its absence. An atmosphere of 10% carbon dioxide was optimal for maximal enterotoxin production.

Enterotoxin was produced by 9 of 10 strains of Clostridium perfringens type A when grown in a defined medium. Additional dextrin increased the amount of enterotoxin in extracts of sporulating cells of strain NCTC 10239.

BACKGROUND Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rheumatoid arthritis. PATIENTS AND METHODS A total of 83 blood samples of patients with RA were s...

AIMS   The aim of this study was to determine whether endophytic Bacillus cereus isolates from agronomic crops possessed genes for the nonhaemolytic enterotoxin (Nhe) and haemolysin BL (HBL) and, therefore, have the potential to cause diarrhoeal illness in humans. METHODS AND RESULTS   PCR followed by sequencing confirmed the presence of enterotoxin genes nheA, nheB, nheC, hblA, hblC, hblD in...

aeromonas hydrophila secretes several extracellular proteins including enterotoxin, hemolysin and aerolysin that are associated with the bacterial virulence. previous studies have shown that two hemolytic toxins, hemolysin a and aerolysin a contribute to the virulence of aeromonas hydrophila. in the current study, a total of 50 strains of aeromonas hydrophila, including 28 (56%) strains isolate...