Background Diarrheal disease is a leading cause of morbidity and mortality globally, especially in low- middle-income countries. High-throughput low-cost approaches to identify etiologic agents are needed guide public health mitigation. Nanoliter-qPCR (nl-qPCR) an attractive alternative more expensive methods yet nascent application without proof-of-concept among hospitalized patients. Methods ...

Background. Infectious diarrhea is a common problem in the developing world, especially among people living with HIV/AIDS. Traditional diagnostic methods such as stool culture and microscopic examination is limited by resources. The use of molecular diagnostics for enteropathogen detection in this region of sub-Saharan Africa has not been fully explored. To identify risk factors and characteriz...

Background. Infectious diarrhea is a common problem in the developing world, especially among people living with HIV/AIDS. Traditional diagnostic methods such as stool culture and microscopic examination is limited by resources. The use of molecular diagnostics for enteropathogen detection in this region of sub-Saharan Africa has not been fully explored. To identify risk factors and characteriz...

Salmonella enterica is a common foodborne, facultative intracellular enteropathogen. Human-restricted typhoidal S . serovars Typhi (STY) or Paratyphi A (SPA) cause severe typhoid paratyphoid fever, while many serovar Typhimurium (STM) strains have broad host range and in human hosts usually lead to self-limiting gastroenteritis. Due restriction of STY SPA primate hosts, experimental systems for...

BACKGROUND Only limited data are available relating to the etiology of diarrhea in children and adults in Senegal. The aim of this prospective study was to describe the epidemiology and etiology of community-acquired diarrheal infections in children and adults living in urban settings. METHODS A prospective study was carried out from March 2009 to December 2010, in the urban region of Dakar, ...

Introduction: Diarrhea infections are caused by various microbial agents such as bacteria, parasites, viruses and fungi. However, in many developing countries, less attention is focused on viral and fungal agents. This work is aimed at determining the viral and fungal agents of diarrhea in children under 5 years of age in Edo State, Nigeria. Methodology: Fecal specimens were collected from diar...

Fe supplementation is a common strategy to correct Fe-deficiency anaemia in children; however, it may modify the gut microbiota and increase the risk for enteropathogenic infection. In the present study, we studied the impact of Fe supplementation on the abundance of dominant bacterial groups in the gut, faecal SCFA concentration and gut inflammation in children living in rural South Africa. In...