PREMISE OF THE STUDY High-throughput sequencing of genomic DNA can recover complete chloroplast genome sequences, but the sequence data are usually dominated by sequences from nuclear/mitochondrial genomes. To overcome this deficiency, a simple enrichment method for chloroplast DNA from small amounts of plant tissue was tested for eight plant species including a gymnosperm and various angiosper...

Listeria monocytogenes is a species of foodborne pathogen often related to foods, such as poultry, ready-to-eat products, fruits, and vegetables. The culture method is a standard procedure for the detection of bacteria in food products. The real-time quantitative PCR (qPCR) technique can be used for the quantification of foodborne pathogens. The current research was aimed to assess...

A new, cost-effective, and environmental-friendly cloud point extraction methodology was described for enrichment of copper, manganese and nickel in several water samples. The method involves the complexation of copper, manganese or nickel with 2-amino-6-(1,3-thiazol-2-diazeyl)-phenol at pH 7.0, then extraction into Triton X-114. After dilution of the surfactant-rich phase with acidified methan...

We report a new method to measure the fraction of glucose derived from gluconeogenesis using gas chromatography-mass spectrometry and positive chemical ionization. After ingestion of deuterium oxide by subjects, glucose derived from gluconeogenesis is labeled with deuterium. Our calculations of gluconeogenesis are based on measurements of the average enrichment of deuterium on carbon 1, 3, 4, 5...

Repetitive and transposable elements comprise more than half of the human genome and play diverse roles in many biological processes. Mobile elements including retrotransposons are implicated in the organization of the epigenetic landscape, the progression of tumorigenesis, and the enhancement of genetic diversity. Despite the importance of repetitive and transposable elements these sequences a...

MOTIVATION Gene set enrichment has become a critical tool for interpreting the results of high-throughput genomic experiments. Inconsistent annotation quality and lack of annotation specificity, however, limit the statistical power of enrichment methods and make it difficult to replicate enrichment results across biologically similar datasets. RESULTS We propose a novel algorithm for optimizi...

The extended finite element method (XFEM) enables the representation of cracks in arbitrary locations of a mesh. We introduce here a variant of the XFEM rendering an optimally convergent scheme. Its distinguishing features are: a) the introduction of singular asymptotic crack tip fields with support on only a small region around the crack tip, the enrichment region, b) only one and two enrichme...

Nonhuman animals in captivity can experience environmental privation that results in their exhibiting abnormal behaviors. Environmental enrichment techniques can help improve their welfare. This study investigated the behavior of 8 zoo-housed oncilla cats (Leopardus tigrinus) in response to 2 odors (catnip and cinnamon) introduced individually into the animals' enclosures for 3 consecutive days...

Group B Streptococcus (GBS) is the most common cause of life-threatening infection in neonates. Guidelines from CDC recommend universal screening of pregnant women for rectovaginal GBS colonization. The objective of this study was to compare the performance of a combined enrichment/PCR based method targeting the atr gene in relation to culture using enrichment with selective broth medium (stand...