We studied the distribution of the alpha 1-alpha 6 subunits of beta 1 integrins in developing and adult human kidney using a panel of mAbs in indirect immunofluorescence microscopy. Uninduced mesenchyme displayed a diffuse immunoreactivity for only the alpha 1 integrin subunit. At the S-shaped body stage of nephron development, several of the alpha subunits were characteristically expressed in ...

BACKGROUND Cerebral amyloid angiopathy (CAA) refers to the deposition of β-amyloid (Aβ) peptides in the wall of brain vasculature, commonly involving capillaries and arterioles. Also being considered a part of CAA is the Aβ deposition in leptomeninge. The cellular origin of angiopathic Aβ and the pathogenic course of CAA remain incompletely understood. METHODS The present study was aimed to e...

Fluid transport across epithelial and endothelial barriers occurs in the neonatal and adult lungs. Biophysical measurements in the intact lung and cell isolates have indicated that osmotic water permeability is exceptionally high across alveolar epithelia and endothelia and moderately high across airway epithelia. This review is focused on the role of membrane water-transporting proteins, the a...

introduction: in the present study, the effects of light cure on wound healing of mice’s skin with complete thickness were studied. materials and methods: forty male mice of nmri race were placed randomly into two groups of examination and control. under aseptic and unconscious conditions, a circular wound of 6 mm width with complete thickness of skin was made on the back of each mice. the surg...

in the development of neuroinflammatory diseases, alterations of the blood brain barrier (bbb) represent key events. the integrity of the bbb is partially maintained by endothelia cells (ecs), since they actively limit the transmigration of immune cells. however, the factors that cause endothelial cells to develop an immune cell-permissive phenotype are poorly understood. in general, it has bee...

PGT is a broadly expressed transporter of prostaglandins (PGs) and thromboxane that is energetically poised to take up prostanoids across the plasma membrane. To gain insight into the function of PGT, we generated mouse monoclonal antibody 20 against a portion of putative extracellular loop 5 of rat PGT. Immunoblots of endogenous PGT in rat kidney revealed a 65-kDa protein in a zonal pattern co...

Predescu, Dan, Stephen M. Vogel, and Asrar B. Malik. Functional and morphological studies of protein transcytosis in continuous endothelia. Am J Physiol Lung Cell Mol Physiol 287: L895–L901, 2004; doi:10.1152/ajplung.00075. 2004.—Continuous microvascular endothelium constitutively transfers protein from vessel lumen to interstitial space. Compelling recent biochemical, ultrastructural, and phys...

Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. Here we report on its extraglomerular localization, using a monospecific antibody which was obtained by affinity purification of IgG on nitrocellulose transfers of glomerular podocalyxin. By indirect immunofluorescence, podocalyxin was found in the blood vessels of several organs (lung, heart, kidney, small intesti...

1. There is a practically constant desquamation of endothelial cells into the circulating blood in rabbits and man. 2. These endothelial cells represent the entire range in size of endothelium from very small cells, possibly from capillaries, up to the type of the large Kupffer cell. 3. These desquamated cells can be discriminated from monocytes. 4. There are common morphological and functional...