Knowledge on the morphology of the cycliophoran female has mostly been based on observations of immature females in brood chambers of feeding stages. With the use of light- and transmission electron microscopy, the morphology and ultrastructure of the free and fully mature female of Cycliophora is described now for the first time. The external morphology is characterized by a ciliation consisti...

Leber congenital amaurosis is a severe hereditary retinal dystrophy responsible for neonatal blindness. The most common disease-causing mutation (c.2991+1655A>G; 10-15%) creates a strong splice donor site that leads to insertion of a cryptic exon encoding a premature stop codon. Recently, we reported that splice-switching oligonucleotides (SSO) allow skipping of the mutant cryptic exon and the ...

Before septation processes shape its four chambers, the embryonic heart is a straight tube that spontaneously bends and twists breaking left-right symmetry. In particular, sub...

Abstract It is estimated that 17.3% of all pregnancies in the horse are lost from time detection to day 40, which has significant economic impact. But still, equine reproductive research limited funding opportunities. Understanding dynamics between embryo, endometrium, and corpus luteum (CL) essential ameliorate those losses. Pig embryonic interferon (IFN) gamma for establishment pregnancy. In ...

clinical reproductive centers produce large amounts of surplus poor-quality embryos annually, how to maximize the use of these embryos, and which of them have the potential to develop into blastocyst stage and influencing factors were lack of systematic research. objective: to investigate the fate of surplus poor-quality embryos which were cultured to obtain blastocyst, determine the factors wh...

objective(s): sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. the present study was designed to analyze the effect of sertoli cells in differentiation of mouse embryonic stem cells (mescs) to germ cells.   materials and methods: a fusion construct composed of a stra8 gene promoter and the coding region of enhanced green fluorescence pro...

Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultur...

Multiciliated cell (MCC) differentiation involves extensive organelle biogenesis required to extend hundreds of motile cilia. Key transcriptional regulators known to drive the gene expression required for this organelle biogenesis are activated by the related coiled-coil proteins Multicilin and Gemc1. Here we identify foxn4 as a new downstream target of Multicilin required for MCC differentiati...