Prior to ICSI the cumulus-oophorous-complex of the oocyte is commonly remove with impure animal-derived hyaluronidase of low strength. The lower strength of the enzyme, the high concentration of impurities and the necessity of extensive time-dependent mechanical denudation damages of the oocytes, increases the risk of lysing the oocytes and the transfer of animal-derived pathogens. All this can...

background: successful embryo transfer (et) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. genetic ...

BACKGROUND Previously manual human embryology in many in vitro fertilization (IVF) centers is rapidly being replaced by closed embryo incubation systems with time-lapse imaging. Whether such systems perform comparably to manual embryology in different IVF patient populations has, however, never before been investigated. We, therefore, prospectively compared embryo quality following closed syste...

Aim: This study was conducted to explore the effect of serum anti-Mullerian hormone (AMH) level on in vitro fertilization (IVF) cycle outcomes. Material and Methods: A total 142 patients included this study, were divided into three groups according their AMH levels as Group 1: 5-10 ng/ml (n=108), 2: 10-15 (n=20), 3: >15 (n=14). Demographic characteristics recorded. The duration infertili...

Background One determining factor of a successful IVF cycle is embryo quality. The aim of the present prospective study was to evaluate the associations between markers of ovarian reserve and ovarian response and embryo quality MaterialsAndMethods One hundred and twenty infertile women, aged 21-44 years, undergoing routine exploration during an unstimulated cycle that preceded ART at our center...

introduction: to evaluate and compare efficiency of sil-select with percoll to recover sperm with normal chromatin in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. materials and methods: assessment of semen parameters, protamine deficiency (cma3) and excessive histone (aniline blue staining) were carried out before and after sperm proces...