Assisted reproductive technologies (ART) are associated with several complications including low birth weight, abnormal placentation and increased risk for rare imprinting disorders. Indeed, experimental studies demonstrate ART procedures independent of existing infertility induce epigenetic perturbations in the embryo and extraembryonic tissues. To test the hypothesis that these epigenetic per...

Large-scale global efforts are underway to knockout each of the approximately 25,000 mouse genes and interpret their roles in shaping the mammalian embryo. Given the tremendous amount of data generated by imaging mutated prenatal mice, high-throughput image analysis systems are inevitable to characterize mammalian development and diseases. Current state-of-the-art computational systems offer on...

Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...

Initiator methionine tRNA (tRNAIMet) species from wheat embryo has been purified by fractionation on a benzoylated DEAE-cellulose column followed by chromatography on a DEAE-Sephadex column. Transfer RNAIMet can be aminoacylated by the homologous enzyme as well as by Escherichia coli aminoacyl-tRNA synthetase. Unlike other eukaryotic cytoplasmic initiator tRNAs, the wheat embryo initiator tRNA ...

The first report of replication of Riley's plasma enzyme-elevating virus (Riley virus) in tissue culture was by Yaffe (1), 2 years after the discovery of the virus in 1960 (2). Yaffe found that Riley virus multiplied in primary mouse embryo cultures until the death of the cultures through overcrowding (3 to 4 weeks), without producing cytopathic or other detectable changes, and could be seriall...

A study was made of the replication of Riley virus in various tissue cultures. It was observed that Riley virus replicated in primary mouse embryo cultures for 8 to 12 days. As they aged primary cultures became less susceptible to Riley virus infection, and subcultured cells were not susceptible. Suspensions of virus, in the absence of cells, were inactivated at 36.5 degrees C. Serial passage b...

Cholesterol is essential for antenatal development. However, the transport of maternal cholesterol to the embryo has not been sufficiently studied, and that to the fetus is still controversial. To this end, a 1 mg dose of [3,4-(13)C(2)]cholesterol was injected daily into pregnant mice and the labeled cholesterol was measured by gas chromatography-mass spectrometry. After venous injections from ...

Studies of receptor-mediated lipoprotein metabolic pathways in avian species have revealed that physiological intricacies of specific cell types are highly analogous to those in mammals. A prime example for the power of comparative studies across different animal kingdoms, elucidated in the chicken, is that the expression of different lipoprotein receptors in somatic cells and oocytes are the k...

The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cu...