The interaction between the HIV-1 transactivator protein Tat and TAR (transactivation responsive region) RNA, plays a critical role in HIV-1 transcription. Iron(II) supramolecular helicates were evaluated for their in vitro activity to inhibit Tat-TAR RNA interaction using UV melting studies, electrophoretic mobility shift assay, and RNase A footprinting. The results demonstrate that iron(II) s...

assays for prostate cancer screening, Afssaps has conducted, in the context of its mission, a market survey of devices indicating quantitative results. The objective of this survey was to evaluate the analytical performances of these assays, particularly to verify the accuracy of total PSA, free PSA and complexed PSA assay kits and to study the equimolarity of total PSA reagents in the analogou...

Quantitative determination of dissociation constants for DNA-protein complexes will help clarify the molecular mechanisms of transcription, replication and DNA repair. A practical capillary electrophoresis mobility shift assay (CEMSA) for protein-DNA affinities free in solution is presented. The method is fast and simple, precise and general. The speed (<2 min separations) and simplicity derive...

A series of forcible curved circular DNAs (cDNAs) were prepared to investigate the recognition features of human Topoisomerase I (hTopo I). The IC(50) can be modulated by the curvature degrees of cDNA. In addition, preferential bindings of hTopo I to cDNA with high curvature have been observed by AFM and EMSA.

Cytokine activation of gene expression can be mediated through signal transducer and activator of transcription (STAT) signaling pathways resulting in expression of target genes. Because many cytokines have important regulatory roles during early development, we wanted to ascertain whether STAT signaling was also active at this time and could therefore have important roles in mediating developm...

1. Introduction Logic Built-In Self-Test (BIST) schemes based on STUMPS structure use on-chip circuitry to generate test stimuli and analyze test responses, with little or no help from an ATE. The STUMPS (Self-Test Using a MISR and Parallel Shift register sequence generator) structure applies pseudo-random patterns generated by a PRPG (Pseudo-Random Pattern Generator) to a full-scan circuit in ...

In today’s Integrated Circuits (ICs), Built-In-Self Test (BIST) is becoming increasingly important as designs become more complicated. BIST is a design technique that allows a circuit to test itself Test pattern generator (TPG) using Linear Feedback Shift Resister (LFSR) is proposed which is more suitable for BIST architecture.In this paper we have design ALU (arithmetic and logic unit) in VHDL...