A novel experimental methodology based on a Prussian blue (PB) and gold nanoparticles (AuNPs) modified carbon ionic liquid electrode (CILE) was developed for use in a label-free amperometric immunosensor for the sensitive detection of human immunoglobulin G (HIgG) as a model protein. The CILE was fabricated by using the ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate as binder. Con...

In this work, we presented a ratiometric electrochemical immunosensor based on redox substrate and immunoprobe. Carboxymethyl cellulose-Au-Pb2+ (CMC-Au-Pb2+) and carbon-Au-Cu2+ (C-Au-Cu2+) nanocomposites were firstly synthesized and implemented as redox substrate and immunoprobe with strong current signals at -0.45 V and 0.15 V, respectively. Human immunoglobulin G (IgG) was used as a model ana...

An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L(-1 )for fumonis...

We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb(2)O(5)) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb(2)O(5) were used to obtain a powerful signal amplification of the electrochemical immunobiosenso...

Acute kidney injury is among the most severe health problems today, with greatest fatality ratios. The molecule-1 (KIM-1) considered to be a potential biomarker for diagnosis of acute injury. Herein, sensitive, selective, and swift sandwich-type electrochemical KIM-1 immunosensor was fabricated based on porous NiCo2S4@CeO2 microspheres as signal amplifier covalent organic frameworks-gold nanopa...

Electrochemical immunosensors have emerged as a highly sensitive and rapid technique for chemical contaminants detection in environmental monitoring, food safety and quality control. This review concentrates on recent advances made in detection and quantification of chemical contaminants such as pesticides and antibiotics residues with electrochemical immunosensors. In this paper, the preparati...

By directly coordinating hippuric acid (HA) to the ferrate (Fe) as an electron transfer mediator, we synthesized a Fe-HA complex, which shows a good electrochemical signal and thus enables the electrochemical immunoanalysis for HA. We electrodeposited organic films containing imidazole groups on the electrode surface and then bonded Ni ion (positive charge) to induce immobilization of Fe-HA (ne...

Gold nanowires functionalized for electrochemical immunosensing were prepared and demonstrated to detect prostate specific antigen (PSA) with the sensitivity required in molecular and clinical diagnostics.