This paper reports a methodology for fast analysis of two important antioxidants: tyrosol and oleuropein in real samples of alimentary oils by using a ready-to-use portable microfluidic system (MicruX ® iHVStat) based on microchips electrophoresis with electrochemical detection. The portable system integrates for the first time a high voltage power supply and a bipotentiostat for using microflu...

In this study, electrochemical molecularly imprinted sensors were fabricated and used for the determination of ascorbic acid (AA). Nanofiber membranes of cellulose acetate (CA)/multi-walled carbon nanotubes (MWCNTs)/polyvinylpyrrolidone (PVP) (CA/MWCNTs/PVP) were prepared by electrospinning technique. After being transferred to a glass carbon electrode (GC), the nanofiber interface was further ...

Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α) - a key inflammatory cytokine - in whole human blood. When testing spiked blood, a TNF-α detect...

The small molecules NO and CO are known to mediate many physiological processes related to neurology , immunology, and muscle cell action. Electrochemical detection is often considered one of the most sensitive methods of detection, in our case exceeding nanomolar concentrations. In this study we married the technology of silicon planar with self-assembled monolayer membrane modified electrodes...

An electrochemical nucleic acid (NA)-based biosensor is a biosensor that integrates a nucleic acid as the biological recognition element and an electrode as the electrochemical signal transducer. The present report provides concepts, terms, and methodology related to biorecognition elements, detection principles, type of interactions to be addressed, and construction and performance of electroc...

In this contribution a chip-integrated amperometric sensor for the detection of H2O2 in exhaled breath condensate (EBC) is reported. The electrode chip is characterized, and detection of H2O2 in an aqueous phase is shown by means of cyclic voltammetry (CV) and amperometry. Variation of conditions such as the composition of the supporting electrolyte largely influences the obtained electrochemic...

The electrochemical studies on the behaviors and recognition of DNA have attracted considerable attention. DNA biosensors based on nucleic acid hybridization process are rapidly being developed towards the goal of rapid and inexpensive diagnosis of genetic and infectious diseases. This brief review focuses on the current state of the DNA electrochemical sensors with emphasis on recent advances,...

This work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen o...

A novel and highly sensitive electrochemical method for the detection of pyritinol in pharmaceutical products and serum samples has been accomplished based on voltamperometric response of pyritinol in carbon nanofiber-gold nanoparticle (CNF-GNP)-modified screen-printed carbon electrode (SPCE). The electrochemical response of pyritinol to CNF-GNP-modified SPCE was studied by cyclic voltammetry a...

Carbohydrate antigen 19-9 (CA19-9) is an important biomarker for the early diagnosis and clinical monitoring of pancreatic cancer. Reliable, simple, and accurate methods for the detection of CA19-9 are still urgently needed. In this study, polythionine-Au composites (AuNPs@ PThi) were designed and prepared through one-pot reaction using HAuCl4 as the co-oxidant and raw material in thionine solu...