Leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. Leptin and itsreceptor (Ob-R) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. The goal of this study was determination of the presence of leptin receptor mRNA in the ram testis, epididymis and spermatozoa by reverse transcription polymerase ...

Male infertility is, perhaps, one of the most complex and poorly understood disease processes in medicine. While some causes of male infertility are clear, the overwhelming majority, remain unknown. Our current treatment approach is to optimize male lifestyle/exposures, endocrine profile, varicocele status (where appropriate) and retrieve sperm or relieve obstruction. One analogy that is freque...

We report that the Gm7068 (CatSpere) and Tex40 (CatSperz) genes encode novel subunits of a 9-subunit CatSper ion channel complex. Targeted disruption of CatSperz reduces CatSper current and sperm rheotactic efficiency in mice, resulting in severe male subfertility. Normally distributed in linear quadrilateral nanodomains along the flagellum, the complex lacking CatSperζ is disrupted at ~0.8 μm ...

Background: CAPZA3 is one of the actin-capping proteins believed to be play an important role in controlling of actin polymerization during spermiogenesis and has been shown to be peresent in mature sperm. Immunohistochemistry showed that human CAPZA3 is localized in the neck region of ejaculated sperm, with moderate and faint signals also detected in the tail and post-acrosome region respectiv...

BACKGROUND This study aimed to assess the relationship between apoptosis in human ejaculated spermatozoa, sperm morphology and the novel sperm deformity index (SDI). METHODS Semen specimens from 50 healthy donors were prepared by density-gradient centrifugation followed by incubating the prepared sperm with paramagnetic annexin V-conjugated microbeads and subjecting this to magnetic cell sort...

AIM To report a birth after intracytoplasmic sperm injection (ICSI) of ejaculated spermatozoa from a man with mosaic Klinefelter's syndrome detected by fluorescence in situ hybridization (FISH) analysis. METHODS A 35-year-old man with a normal appearance consulted our hospital because of sterility over a 5-year period. Chromosome analysis showed low-incidence mosaic Klinefelter's syndrome. Us...

Sperm genomic integrity and ultrastructural features of ejaculated spermatozoa contributing to the assessment of gamete fertility potential in patients with asthenozoospermia are discussed. The proportion of TUNEL-positive cells was significantly higher in the semen of patients with low sperm motility (n=40; p<0.01) as compared to men with normal sperm motility (n=54). Sperm DNA fragmentation n...

spermatogenesis and spermiogenesis, mam-malian spermatozoa leaving the testis appear to be morphologically mature but clearly are immature from a functional standpoint; that is, they have acquired neither progressive motility nor the ability to fertilize a metaphase II–arrested egg. Although progressive motil-ity is acquired and signaling pathways mature during sperm transit through the epididy...