صلاخلا ــ ة : مت ةقرولا هذه يف ءاشنإ يف هدعاسملل مدقتم ماظن بناوج ذيفنتو ميمصت ضرع قتو و مي ءادأ حيحصت تارفش لأا ءاطخ ةليلق لا ةناتم ، ةينبم ىلع ءوفاكتلا رايتخا . موقي امه نيتيسيئر نيتفيظوب ةساردلا هذه يف مدقتملا ماظنلا : 1 ادأ باسحل ةيزاوتملا هاآاحملا ء تارفش حيحصت لأا ءاطخ ةليلق نمزب ةفاثكلا ادج ريصق ةنراقم ةمظنأب ةاآاحملا ةدوجوملا . 2 ةليلق ءاطخلأا حيحصت تارفشو فعضلا قطانم صحف نع ة...

The effect of Ca2+ on guinea pig bra in adenylate cyclase has been studied using metal buffers to regulate the free Ca2+ concentration. Particulate fractions prepared in the presence of CaZ+ (to bind all of the available calmodulin to all of the available binding sites) exhibited a biphasic response to Ca2+. The first was a 2fold stimulation of basal cyclase activity (half-maximal = 0.08 p ~ ) ...

Lappaconitine (LA), isolated from Aconitum sinomontanum Nakai, was characterized as analgesic principle by our laboratory. The analgesic effect of ip LA 6 mg/kg as measured in the rat tail-flick test was reduced by icv CaCl2 or MgCl2 0.1 or 1 mumol/rat. BaCl2 was inactive. The analgesic action induced by LA was potentiated by ethylene glycol tetraacetic acid (EGTA, 0.2 mumol/rat icv) but not by...

Rotavirus (RV) cores were released from double-layered particles (DLPs) by high concentrations of CaCl2, purified and 'opened' by treatment with EDTA or EGTA. Under appropriate in vitro conditions DLPs have been shown to have transcriptase and 'open cores' replicase activity. Furthermore, it has been demonstrated that transcriptase activity and infectivity of native cores can be restored by tra...

Experiments with plasma membrane from rat liver have been interpreted as showing that the activity of the (Na++K+)-dependent ATPase* is subject to modulation by the action of hormones, such as L-adrenaline (epinephrine) and insulin, through the latter's effects on adenylate cyclase activity and consequent changes in cyclic AMP concentration (Luly et af. , 1972; Tria et af . , 1974; Luly & Emmel...

We examined alternative and classical complement activation induced by whole bacilli of Mycobacterium bovis BCG and Mycobacterium tuberculosis products. After exposure to BCG, there were higher levels of the terminal complement complex in sera from Indian tuberculosis patients than in sera from healthy controls. The addition of BCG with or without EGTA to these sera indicated that approximately...

Treponema hyodysenteriae was incubated in 20% normal swine sera (NSS) at 37 degrees C for 4 h, and viability was determined by a plate dilution method. NSS was bactericidal for nonpathogenic T. innocens and avirulent T. hyodysenteriae, but not for virulent T. hyodysenteriae isolates. Heat inactivation at 56 degrees C for 30 min, treatment with EDTA or EGTA [ethylene glycol-bis(beta-aminoethyl e...

Studies directed at understanding the molecular basis of liver cell homotypic adhesion are presented. An assay which measures the rate of adhesion of isotopically labeled (32PO4) embryonic chick liver cells to liver cell aggregates, described in a companion paper, has been used to investigate the problem of intercellular adhesive selectivity. Cation requirements, the effects of various inhibito...

α-Amylases are widespread enzymes that catalyze endohydrolysis of long α-1,4-glucan chains such as starch and glycogen. The highest amylolytic activity was found in 5th instar nymphs and midgut of the predatory bug, Andrallus spinidens F. (Hemiptera: Pentatomidae). The α-amylase was purified following a three-step procedure. The purified α-amylase had a specific activity of 13.46 U/mg protein, ...