There are limited data regarding the antifungal susceptibility of yeast causing vulvovaginal candidiasis, since cultures are rarely performed. Susceptibility testing was performed on vaginal yeast isolates collected from January 1998 to March 2001 from 429 patients with suspected vulvovaginal candidiasis. The charts of 84 patients with multiple positive cultures were reviewed. The 593 yeast iso...

There is some evidence that the mouse lymphoma TK assay (MLA) can detect aneugens, and this is accepted in the current International Conference on Harmonisation guidance for testing pharmaceuticals. However, whether or not it can be used as a reliable screen for aneugenicity has been the subject of debate. Consequently, aneugens with diverse mechanisms of action were tested in the MLA using 24-...

Azole antifungal drug ketoconazole has recently been demonstrated as an inhibitor of a ligand-induced pregnane X receptor (PXR)-mediated transcriptional regulation of the CYP3A4 gene through disruption of PXR interaction with steroid receptor coactivator (SRC)-1. In contrast, other clotrimazole-derived antifungal agents are known as potent inducers of CYP3A4 through PXR. In the present study, w...

The effect of 3-¿1-(p-chlorobenzyl)-5-(isopropyl)-3-tert-butylthioindol-2-yl-2, 2-dimethylpropanoic acid (MK-886), a leukotriene biosynthesis inhibitor, on Ca(2+) mobilization in Madin- Darby canine kidney cells has been examined by fluorimetry using fura-2 as a Ca(2+) indicator. MK-886 at 0.5 to 25 microM concentration dependently increased ¿Ca(2+)(i). The ¿Ca(2+)(i) increase comprised an imme...

INTRODUCTION. Candida albicans is the most common inhabitant of the skin, mouth, vagina and gastro intestinal tract of human beings. One of the major reasons for the increase in Candida infection is the development of its resistant strains due to drugs used in the treatment of candidiasis. MATERIALS AND METHODS We studied 4027 samples collected from patients in various wards of the Emergency Co...

AIM The management of suppurative keratitis due to filamentous fungi presents severe problems in tropical countries. The aim was to demonstrate the efficacy of chlorhexidine 0.2% drops as an inexpensive antimicrobial agent, which could be widely distributed for fungal keratitis. METHODS Successive patients presenting to the Chittagong Eye Institute and Training Complex with corneal ulcers wer...

In Jurkat and human peripheral blood T-lymphocytes, 1-oleoyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analogue of diacylglycerol, activated the influx of Ca(2+), Ba(2+) and Sr(2+). OAG also caused plasma-membrane depolarization in Ca(2+)-free media that was recovered by the addition of bivalent cation, indicating the activation of Na(+) influx. OAG-induced cation influx was (i) mimicked ...

Yicheng Zhang, Jonathan Soboloff, Ziping Zhu & Stuart A. Berger _____________________________________________________________ From the Arthritis and Immune Disorder Research Centre, University Health Network and the Department of Immunology, University of Toronto; Toronto, Ontario, Canada M5G 1L7 (YZ, JS, ZZ, SAB). Current Address: Department of Hematology, Tongji Hospital, Tongji Medical Colle...

A 41-year-old man presented with a 24 hour history of a painful right eye. Nineteen months previously he had splashed molten lead into this eye. He had subsequently undergone mucous membrane grafting to repair scarred conjunctival fornices. A vascularised cornea remained with associated cicatricial entropion preventing eyelid closure. He underwent further upper fornix reconstruction using mucou...