نتایج جستجو برای: e coli dh5α
تعداد نتایج: 1122935 فیلتر نتایج به سال:
Some VanM-type vancomycin-resistant Enterococcus faecium isolates from China are also resistant to fosfomycin. To investigate the mechanism of fosfomycin resistance in these clinical isolates, antimicrobial susceptibility testing, filter-mating, Illumina/Solexa sequencing, inverse PCR and fosfomycin resistance gene cloning were performed. Three E. faecium clinical isolates were highly resistant...
The present work was conducted to compare the occurrence of Escherichia coli possessing virulence and ESBL genes in backyard and farmed poultry. Three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from West Bengal, India. Among the E. coli isolates of backyard poultry (O2, O10, O25, O55, O60, O106, UT), none of them posse...
background: candida albicans (c. albicans) is a major cause of candidaemia in people with impaired immunity. blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. we established a real-time pcr assay for c. albicans detection in blood by lightcycler pcr and melting curve analysis. methods: five milliliter blood samples from healthy volunt...
Colibacillosis is known as a fatal bacterial disease resulting in a high level of commercial loss worldwide. This study amid to elucidate the sequence, genetic characteristics, and phylogeny of the bor gene in Escherichia coli (E. coli) strain c1378 (O78:K80) isolated from avian colibacillosis in Iran and develop a rapid and optimal polymerase chain reaction (PCR) mol...
هدف از این تحقیق کلون کردن ژن سازنده ناحیه ثابت زنجیره سنگین igg گاو و بررسی بیان آن در e. coli بود. بدین منظور ابتدا توالی نوکلئوتیدی ژن سازنده ناحیه ثابت زنجیر gamma 1 گاو از بانک ژن استخراج و مورد بررسی قرار گرفت تا پرایمرهای مناسب برای تکثیر این ژن در آزمایش rt-pcr طراحی گردد. سپس با استفاده از پرایمرهای طراحی شده ژن سازنده ناحیه ثابت زنجیر gamma 1 گاو با روشrt-pcr تکثیر گشت. در این آزمایش ...
Enterohemorrhagic Escherichia coli (EHEC) of the O157:H7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human EHEC disease. The aim of the present study was to investigate the prevalence of E. coli O157: H7/NM in raw meat samples from two provinces of Iran. During a period from March 2010 to March 2011. Two hundred and ninety five raw meat samples were...
background: targeting of cd20 antigen with monoclonal antibodies has become the mainstay in the treatment of non-hodgkin's lymphomas and immunotherapeutic depletion of malignant b cells. accessibility of antigen is one of the crucial factors in development of monoclonal antibodies against this antigen. one major problem in expression of full length cd20 is aggregation and misfolding. there...
nanotechnology is a new and specific method for therapy of diseases. nanosilver particle is one of the functional fields of nanotechnology. it has been shown that these nanoparticles have antibacterial and antifungal properties. different studied have been shown that nanoparticles may have important role in medicine. for example, it has been shown to have inhibitory effects on the treatment of ...
the present work was conducted to compare the occurrence of escherichia coli possessing virulence and esbl genes in backyard and farmed poultry. three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from west bengal, india. among the e. coli isolates of backyard poultry (o2, o10, o25, o55, o60, o106, ut), none of them posse...
background and objectives: diarrheagenic escherichia coli (dec) is an emerging agent among pathogens that causes diarrhea. studies showed that diarrheagenic e. coli such as enterohaemorrhagic e. coli (ehec), enteroaggregative e. coli (eaec), enteropathogenic e. coli (epec), enteroinvasive e. coli (eiec), enterotoxigenic e. coli (etec), diffusely adhering e. coli (daec) and shiga toxin producing...
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