نتایج جستجو برای: duplex rt pcr

تعداد نتایج: 214870  

2010
Ying Wang Ying-Li Cao Fan Yang Yun Zhang Shu-Hui Wang

Small interfering RNA (siRNA) is a class of duplex RNA molecules of 21-25 nt nucleotides in length functioning post-transcriptionally to downregulate targeted gene expression. The membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is highly abundant during viral infections and is a critical element for viral assembly. Nucleotide substitution in the viral...

Journal: :BioTechniques 1998
L L Hall G R Bicknell L Primrose J H Pringle J A Shaw P N Furness

The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...

Journal: :Molecules 2010
Ying Wang Ying-Li Cao Fan Yang Yun Zhang Shu-Hui Wang Li Liu

Small interfering RNA (siRNA) is a class of duplex RNA molecules of 21-25 nt nucleotides in length functioning post-transcriptionally to downregulate targeted gene expression. The membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is highly abundant during viral infections and is a critical element for viral assembly. Nucleotide substitution in the viral...

Journal: :European Journal of Obstetrics & Gynecology and Reproductive Biology 2021

ObjectiveTo screen pregnant women at risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during delivery using reverse-transcription polymerase chain reaction (RT-PCR) test and serum immunoglobulin (Ig) testing.MethodBetween March 31 st August 2020, consecutive admitted for labor in a single hospital were screened SARS-CoV-2 with nasopharyngeal RT-PCR swab tests detec...

Journal: :iranian journal of microbiology 0
f abbasian virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. h tabatabaie virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. m sarijloo virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. s shahmahmoodi virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. a yousefi virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. t saberbaghi virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran.

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

Journal: :medical journal of islamic republic of iran 0
m azarnoosh from the department of biotechnology, pasteur institute of iran, tehran, islamic republic of iran. s zeinali sm tabatabaei a ziaee

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

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