The polymerase chain reaction (PCR) was investigated for detecting human parvovirus B19 (B19) DNA in sera. Three pairs of oligonucleotides were evaluated as primers. The best oligonucleotide pair spanned 699 nucleotides, including the region common to VP1 and VP2. After PCR amplification of B19 DNA in serum, a 699-nucleotide DNA fragment was detected on agarose gels. This DNA fragment was B19 D...

Cherax destructor occurs naturally and/or is farmed in all Australian mainland states and territories and is of major cultural, economical and conservation significance. The aim of this study was to determine susceptibility of the commercially important subspecies C. destructor albidus to white spot syndrome virus (WSSV), a hazard to crustaceans and currently considered to be exotic to Australi...

We describe a simple and rapid dot immunoperoxidase assay for the direct detection of parvovirus B19 capsid antigens in human sera. The assay was performed with serum specimens dotted onto nylon membranes. VP1 and VP2 B19 antigens, which represent 4 and 96% of the capsid, respectively, were detected with a pool of monoclonal antibodies directed against the two proteins, and the complex was visu...

BACKGROUND Eosinophilic meningitis caused by Parastrongylus cantonensis, the rat lung worm is a major public health problem in Thailand. Humans acquire this parasite by eating raw food containing infective Parastrongyliasis is dificult to make because identification of parasite materials by biopsy or chance finding is rarely possible. OBJECTIVE Develop alternative approaches of Parastrongylus...

Tannins have received considerable attention from animal nutritionists as potential agents for modifying ruminal fermentation patterns, or for exploring new feed resources. This group of secondary plant compounds is defined by their ability to form complexes with proteins. A widely accepted method for assaying the biological activity of extracted tannins is the precipitation of bovine serum alb...