Annotation. The problem of effective and safe treatment pain syndromes is a priority area modern medicine, since syndrome often accompanied by economic costs, drop in labor productivity an increase the risk other diseases. derivative 4-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxamide (compound NI-9) has shown significant analgesic effect various models syndromes. aim this work was to exper...

Whilst astrocytes in culture invariably respond to dopamine with cytosolic Ca2+ rises, the dopamine sensitivity of astroglia in situ and its physiological roles remain unknown. To minimize effects of experimental manipulations on astroglial physiology, here we monitored Ca2+ in cells connected via gap junctions to astrocytes loaded whole-cell with cytosolic indicators in area CA1 of acute hippo...

Intraperitoneal (IP) injection of bupropion (3,6, amine (4,16 mg kg•') induced dose-dependent climbing in mice. The climbing response induced by both drugs were decreased in animals pretreated either with the 0-1 antagonist SCH 233<)0 or the 0-2 antagonist sulpiride. The α-adrenoceptor blocker phenoxybenzamine decreased the climbing induced by both bupropion and amphetamine, but the β-ad...

Solubilization of dopamine receptors, in a native form, was first achieved in our laboratory using dog striatum and the mild detergent digitonin [l-3]. These results were confirmed by another group in both dog and human brain [4f. However, earlier attempts to solubilize dopamine receptors from rat striatum were ~s~essfui~ even though a similar experimental procedure was used [3,5]. Although the...

We identified protein 4.1N as a D2-like dopamine receptor-interacting protein in a yeast two-hybrid screen. Protein 4.1N is a neuronally enriched member of the 4.1 family of cytoskeletal proteins, which also includes protein 4.1R of erythrocytes and the 4.1G and 4.1B isoforms. The interaction of protein 4.1N was specific for the D2 and D3 dopamine receptors and was independently confirmed in pu...

The progressive degeneration of the dopamine neurons of the pars compacta of substantia nigra and the consequent loss of the dopamine innervation of the striatum leads to the impairment of motor behavior in Parkinson's disease. Accordingly, an efficient therapy of the disease should protect and regenerate the dopamine neurons of the substantia nigra and the dopamine innervation of the striatum....

Current thought envisions dopamine neurons conveying the reinforcing effect of the unconditioned stimulus during associative learning to the axons of Drosophila mushroom body Kenyon cells for normal olfactory learning. Here, we show using functional GFP reconstitution experiments that Kenyon cells and dopamine neurons from axoaxonic reciprocal synapses. The dopamine neurons receive cholinergic ...

Salivary secretion is crucial for successful tick feeding, and it is the mediator of pathogen transmission. Salivation functions to inhibit various components of the host immune system and remove excess water and ions during the ingestion of large blood meals. Control of salivary glands involves autocrine/paracrine dopamine, which is the most potent inducer of tick salivation. Previously, we re...

The cloning of the D2 dopamine receptor (6) in 1988 and the subsequent identification of multiple dopamine receptors referred to as D1, D3, D4, and D5 (10, 35, 46, 48, 49, 52, 54, 59) has profoundly changed our understanding of dopamine receptor anatomy and pharmacology. Prior to the isolation of these dopamine receptor subtypes, the dopamine field distinguished two subtypes of dopamine recepto...

Five subtypes of dopamine receptor exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). We produced novel monoclonal antibodies against all three D2-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most of the immunostaining for all three receptors colocalized with mannose-6-phosphate receptor, a ...