It is well known that ionizing radiation (IR) can damage DNA through a direct action, producing single- and double-strand breaks on DNA double helix, as well as an indirect effect by generating oxygen reactive species in the cells. Mammals have evolved several and distinct DNA repair pathways in order to maintain genomic stability and avoid tumour cell transformation. This review reports import...

background: colorectal cancer is the third cause of cancer death in western countries. age, inadequate diet, obesity, inactivity and genetic changes are some of the risk factors of colorectal cancer. correlation of genetic diversity in homologous recombination repair system with cancer was evaluated in many recent studies. this study was done to investigate the correlation of t241m polymorphism...

this study has examined the relationship between the xpd lys 751 gln polymorphism and colorectal cancer in 88 patients and their 88 age and sex-matched controls. genomic dna from peripheral whole blood was extracted using standard method to determine the genotype of subjects with rflp-pcr analysis. although this study shows cancer patients harbor more heterozygous genotype (xpd lys 751 gln) (or...

• Molecular response to chemo-, radio- and radionuclide therapy was assessed in NENs. Cisplatin PRRT triggered DNA damage repair-related gene expression. Bortezomib inhibited repair related Combined resulted short-term pro-apoptotic effects vitro . Tumoral heterogeneity interfered with long-term sensitization assessment vivo Background: Well-differentiated gastroenteropancreatic neuroendocrine ...

In eukaryotes, multiple genes encode histone proteins that package genomic deoxyribonucleic acid (DNA) and regulate its accessibility. Because of their positive charge, 'free' (non-chromatin associated) histones can bind non-specifically to the negatively charged DNA and affect its metabolism, including DNA repair. We have investigated the effect of altering histone dosage on DNA repair in budd...

The creation of a DNA break at a specific locus by a designer endonuclease can be harnessed to edit a genome. However, DNA breaks may engage one of several competing repair pathways that lead to distinct types of genomic alterations. Therefore, understanding the contribution of different repair pathways following the introduction of a targeted DNA break is essential to further advance the safet...

Long non-coding RNAs (lncRNAs) play important roles in regulating diverse biological processes including DNA damage and repair. We have recently reported that the DNA damage inducible lncRNA DNA damage-sensitive RNA1 (DDSR1) regulates DNA repair by homologous recombination (HR). Since lncRNAs also modulate gene expression, we identified gene expression changes upon DDSR1 knockdown in human fibr...

The uvrD252 mutation leads to increased UV sensitivity, diminished dimer excision and host cell reactivation capacity, and an increase in the average patch size after repair replication. A recA56 uvrD252 double mutant was far more resistant to UV than was a recA56 uvrB5 double mutant. Its host cell reactivation capacity was identical to that of uvrD252 single mutant and was far greater than tha...

We recently identified a positional candidate for the XRCC2 DNA repair gene at human chromosome 7q36.1. We have now cloned the cDNA for this gene from both human and mouse and show that it is a highly conserved novel member of the recA / RAD51 recombination repair gene family. The cDNA is able to complement significantly the phenotype of a unique cell line, irs1 , which shows extreme sensitivit...