Characterization of predator-prey interactions is challenging as researchers have to rely on indirect methods that can be costly, biased and too imprecise to elucidate the complexity of food webs. DNA amplification and sequencing techniques of gut and fecal contents are promising approaches, but their success largely depends on the ability to amplify the taxonomic array of prey consumed and the...

Cassia angustifolia Vahl. is native to Saudi Arabia. This shrub has been used as a laxative and even fungicide. The current study was designed evaluate the morphology molecular characterization of plants from two areas in Purified genomic DNA subjected PCR amplification using random primers (RAPD). showed that, all morphological parameters were statistically significant (P ≤ 0.05) when compa...

faced with the problem that only a partial protein sequence is known. Several procedures are commonly used involving the reverse translation of the protein sequence into a DNA sequence. These include synthesis of a short oligonucleotide sequence for screening libraries and of a primer pair for amplification of target sequence by polymerase chain reaction (PCR). However, due to redundancy in the...

Name of Gene Marker. MPO, myeloperoxidase. Source and Description of Primers. Primers (set A) were designed based on human (Genbank Accession no. D14466) and mouse (Genbank Accession no. X15313) MPO exon 9 and 10 sequences available in the Genbank database. Using porcine genomic DNA as template in the PCR, these primers amplified a 1-kb fragment. Set B primers were designed based on pig DNA seq...

Primers for the amplification and sequencing of DNA fragments from chloroplast genes and non-coding regions are provided to facilitate molecular phylogenetic studies aimed at building a tree-of-life for the Asteraceae. The primers reported here have been extensively tested and some empirical guidelines are included to facilitate their use.

A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin resistance gene on the vector and reverts the vector to ampicillin resistance. Using T4 DNA polymer...

A rapid and versatile method has been developed for the synthesis of oligonucleotides which contain an aliphatic amino group at their 5' terminus. This amino group reacts specifically with a variety of electrophiles, thereby allowing other chemical species to be attached to the oligonucleotide. This chemistry has been utilized to synthesize several fluorescent derivatives of an oligonucleotide ...

Name of Gene Marker. MBL2, Mannose-Binding Lectin 2 (Mannose-Binding Protein, Serum; MBP1). Source and Description of Primers. Primers (set A) were designed based on human (GenBank accession number X15422) and cattle (GenBank accession number D73408) MBL2 sequences available in the GenBank database. Using porcine genomic DNA as a template in the PCR, these primers amplified an 831-bp fragment. ...