Phenol emulsion reassociation technique (PERT) greatly enhances the rate of DNA hybridization in solution over typical hybridization conditions (1) and has successfully been used to accelerate competitive DNA reassociation for performing subtraction based genomic cloning (2-5). PERT is accomplished through the establishment and maintenance of a phenol emulsion, typically by mechanical vortexing...

Case presentation: Comparative genomic hybridization based on microarrays (array CGH) is a reality in clinical practice the neuropediatric population. It allows high-resolution assessment of DNA copy number changes associated with chromosomal abnormalities. Objective: To highlight importance using technique investigation patients diverse phenotypes. Methods: Series case studies.

The development of biosensors for detection and identification of DNA sequences by hybridization may reduce assay time and allow direct quantitation of the target. This article describes the use of impedance spectroscopy technique for digoxigeninthiol-labeled ssDNA probe immobilization, anti-digoxigenin binding and targeted DNA hybridization. The analysis of the impedance spectra in terms of eq...