DNA mimic proteins have DNA-like negative surface charge distributions, and they function by occupying the DNA binding sites of DNA binding proteins to prevent these sites from being accessed by DNA. DNA mimic proteins control the activities of a variety of DNA binding proteins and are involved in a wide range of cellular mechanisms such as chromatin assembly, DNA repair, transcription regulati...

Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving replication, recombination, and repair all living beings. In molecular biology, SSB are used as enhancers to increase the efficiency specificity of PCR. Thermostable protein eliminates secondary structure or dimer formation significantly effectiveness amplification fragments. this study, it was ensured...

Protein-DNA interactions are the core of cell’s molecular machinery. For a long time, conventional biochemical methods served as powerful investigatory basis protein-DNA and target search mechanisms. Currently single-molecule (SM) techniques have emerged complementary tool for studying these revealed plenty previously obscured mechanistic details. In comparison to traditional ones, SM allow dir...

The gram-positive bacterium Bacillus licheniformis exhibits obvious selective utilization on carbon sources. This process is mainly governed by the global regulator catabolite control protein A (CcpA), which can recognize and bind to multiple target genes that are widely distributed in metabolic pathways. Although DNA-binding domain of CcpA has been predicted, influence key amino acids gene rec...

Pathogens secrete effector proteins into host cells to suppress immunity and promote pathogen virulence, although many features at the molecular interface of host–pathogen interactions remain be characterized. In a yeast two-hybrid assay, we found that Pseudomonas syringae HopZ1a interacts with Arabidopsis transcriptional regulator Abscisic Acid Repressor 1 (ABR1). Further analysis revealed ABR...

air pollutants are reported to increase the specific ige response to allergens. to study allergen proteins and determine the effect of air pollutants on the allergenic potential of pollen extracts, the extracts were injected intraperitoneally and subcutaneously in guinea pigs. the results were analyzed by skin prick test (spt), elisa and sds-page followed by immunoblotting. the pollen extract o...

Cys2His2 zinc finger proteins are important for living organisms, as they—among other functions—specifically recognise DNA when Zn(II) is coordinated to the proteins, stabilising their ??? secondary structure. Therefore, competition with metal ions may alter original function. Toxic such Cd(II) or Hg(II) might be especially dangerous because of similar chemical properties Zn(II). Most studies c...