The monomer-dimer association constant of solubilized and delipidated sarcoplasmic reticulum ATPase was measured by large zone elution gel chromatography in the presence of a high concentration (18.6 mM) of the nonionic detergent dodecyloctaethylene glycol monoether (C12E8) and of different ATPase protein concentrations in the range of 0.74 (6.4 nM monomers) to 30 (0.26 microM monomers) microgr...

Charybdotoxin (CTX), a small, basic protein from scorpion venom, strongly inhibits the conduction of K ions through high-conductance, Ca2+-activated K+ channels. The interaction of CTX with Ca2+-activated K+ channels from rat skeletal muscle plasma membranes was studied by inserting single channels into uncharged planar phospholipid bilayers. CTX blocks K+ conduction by binding to the external ...

The cyclic methane hydrate production method was proposed with CO 2 and N mixture stimulation. model established based on actual reservoir parameters, accordingly, the characteristics were analyzed, a sensitivity analysis conducted. results show following: (1) depressurization mechanism is dominant in production. CH 4 dissociation can be greatly enhanced because process effectively reduce parti...

epidemiological studies and clinical findings have shown an association between trauma and dissociation; dissociative experiences are also more prevalent among select populations such as substance dependent individuals and criminal offenders. in the present non-systematic review, we explored the association between trauma, dissociation, and high-risk behaviors. we aimed to find if the presence ...

Tetramer-dimer equilibrium of lambda repressor has been studied by fluorescence anisotropy techniques. We have chosen 1-dimethylamino naphthalene-5-sulfonyl chloride (dansyl chloride)-labeled repressor to study the dissociation-association equilibrium, because of relatively long life-time of the probe (> 10 ns). Polarization of the dansyl-labeled repressor decreases with decreasing protein conc...

The pH dependence of the apparent tetramer to dimer dissociation constant has been determined at 20 degrees for both oxy- and deoxyhemoglobins A and Kansas. These measurements were made by three different procedures: gel chromatography, sedimentation velocity, and kinetic methods in either of three buffer systems: 0.05 M cacodylate, Tris, or glycine with 1 mM EDTA and 0.1 M NaCl between pH 6.5 ...