The Autobac 1 system was compared to the disk diffusion and agar dilution methods. Overall, there was good correlation; however, certain antibiotic/bacterial combinations gave unreliable results. In particular, unreliable results were obtained against staphylococci with penicillins, aerobic gram-negative rods with cephalothin, and enterococci with cephalothin and clindamycin.

The in vitro inhibitory activity of trimethoprim-sulfamethoxazole is inversely related to the amount of thymine and thymidine in the test medium; manufacturers must adequately control their media to avoid spurious antimicrobial susceptibility results. No differences were observed when commercial Mueller-Hinton broth and agar were compared with a semisynthetic broth medium by the use of microdil...

The agar dilution, broth microdilution, and disk diffusion methods were compared to determine the in vitro susceptibility of 428 extended-spectrum-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin. Fosfomycin showed very high activity against all ESBL-producing strains. Excellent agreement between the three susceptibility methods was found for E. coli, whereas ...

The agar dilution, broth microdilution, and disk diffusion methods were compared to determine the in vitro susceptibility of 428 extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin. Fosfomycin showed very high activity against all ESBL-producing strains. Excellent agreement between the three susceptibility methods was found for E. coli, whe...

Fifty-four motile Aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. Cephalothin susceptibility was significantly associated with Aeromonas sobria (P less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.

A five-center collaborative study was undertaken to develop quality control and specific interpretive criteria for susceptibility testing of Streptococcus pneumoniae against 12 antimicrobial agents. MICs were determined for 248 pneumococcal clinical isolates (with an emphasis on resistant strains) by use of the National Committee for Clinical Laboratory Standards (NCCLS)-recommended broth micro...

orange peel extract solution contains mucilage flavor and three types of glycosides. limonene is one of the terpenoides in some vegetables, fruits and food; that plays as an antioxidant in juice. as it has anti-microorganism effects on some bacteria, we carried out the antifungal effects of limonene versus orange extract. in the present study the effects of orange extract and two types of limon...

In the present study, Anacker and Ordal agar, marine agar (MA), and Flexibacter maritimus medium (FMM) were compared with the dilute versions of Mueller-Hinton agar (DMHA) medium recommended by the National Committee for Clinical Laboratory Standards (NCCLS) for their use in disk diffusion tests with Tenacibaculum maritimum strains and to calculate the MICs of five drugs by the Etest method. Pr...