Abstract Background Evans’ syndrome (ES) is a rare and chronic autoimmune disease characterized by the concomitant or sequential association of auto-immune hemolytic anemia (AIHA) with immune thrombocytopenia (ITP), less frequently neutropenia positive direct anti-human globulin test. ES represents up to 7% AIHA around 2% ITP. Studies have found that coronavirus 2019 (COVID-19) may be associate...

Gamma globulin formation in vitro by various tissues was studied using the incorporation of C(14)-L-lysine into a protein precipitable by a specific anti rabbit gamma globulin serum prepared in a sheep. It was demonstrated that the rate of gamma globulin formation in similar numbers of spleen cells is much higher if taken from an immunized rabbit at the height of antibody formation than that in...

INTRODUCTION Since there is scarce data available on incidence and type of irregular antibodies in patients and donors in India, a study was undertaken to find the incidence of irregular antibodies in patients and irregular antibodies and a positive Direct Antiglobulin Test (DAT) in blood donors. MATERIALS AND METHODS Antibody screening was performed using commercial pooled "O" cells and thre...

SYNOPSIS The indirect fluorescent antibody technique has been used to titrate patients' antibody against Toxoplasma gondii. Rigorous tests of the immunological validity of the method were made. The reactions of the gamma globulin of human immune serum with Toxoplasma gondii and fluorescent rabbit anti-human gamma globulin were thus shown to be specific. Thereafter the technique could be used wi...

The indirect fluorescent antibody technique has been used to titrate patients' antibody against Toxoplasma gondii. Rigorous tests of the immunological validity of the method were made. The reactions of the gamma globulin of human immune serum with Toxoplasma gondii and fluorescent rabbit anti-human gamma globulin were thus shown to be specific. Thereafter the technique could be used with confid...

(2) The L.E. Cell Test.-In about 80 per cent. of the proved cases the Hargraves test is positive. A theoretical objection to this test is that it employs phagocytosis which is a secondary reaction in vitro as indicator. The fixation of the specific L.E. gamma globulin to nuclei, which constitutes the first phase of the L.E. cell formation and results in loose body formation (Aisenberg, 1959; Ri...

The concept that lymphocytes recognize antigens by means of a surface receptor molecule of immunoglobulin (Ig) character has come to be widely accepted (1-3). Direct study of such receptors in the mouse by means of antiglobulin reagents labeled with either fuorescent (4, 5) or radioisotope (3, 6-8) markers has revealed a dense coating of surface immunoglobulins on nonthymus-derived or B lymphoc...