Phenol is one of the main pollutants that have a serious impact on environment and can even be very critical to human health. The biodegradation phenol considered an increasingly important pollution control process. In this study, degradation by Bacillus simplex was investigated for first time under different growth conditions. Six initial concentrations were used as primary substrate. Culture ...

Spheres of influence: Although cysteine and 3-mercaptopropionate dioxygenase bind react similar substrates, there are key differences in the first- second-coordination spheres that affect their catalytic reaction mechanism reactivity, as highlighted by density functional theory studies on large enzyme clusters.

In the mammalian genome, cytosine methylation predominantly occurs at CpG sites. addition, a number of recent studies have uncovered extensive C5 (5mC) non-CpG (5mCpH, where H = A/C/T) Little is known about enzyme responsible for active demethylation 5mCpH Using very sensitive and quantitative LC–MS/MS method, we demonstrate that human TET2, an iron (II)- 2OG-dependent dioxygenase, which freque...

Burkholderia sp. strain AK-5 utilized 4-aminophenol as the sole carbon, nitrogen, and energy source. A pathway for the metabolism of 4-aminophenol in strain AK-5 was proposed based on the identification of three key metabolites by gas chromatography-mass spectrometry analysis. Strain AK-5 converted 4-aminophenol to 1,2,4-trihydroxybenzene via 1,4-benzenediol. 1,2,4-Trihydroxybenzene 1,2-dioxyge...

Introduction The enzymes tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO1) catalyse oxidation of the essential amino acid tryptophan (Trp) leading to the formation of immunosuppressive kynurenine (Kyn) pathway metabolites that dampen the immune response in the tumour microenvironment. Both IDO1 and TDO have been shown to be up-regulated in a variety of cancers and blockade...

The Saccharomyces cerevisiae open reading frame YLL057c is predicted to encode a gene product with 31.5% amino acid sequence identity to Escherichia coli taurine/alpha-ketoglutarate dioxygenase and 27% identity to Ralstonia eutropha TfdA, a herbicide-degrading enzyme. Purified recombinant yeast protein is shown to be an Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase. Although taurin...