نتایج جستجو برای: dimensional gel electrophoresis

تعداد نتایج: 509153  

Journal: :Biochemical and biophysical research communications 2007
Guihua Shi Tianzi Jiang Wanlin Zhu Bing Liu Huizhi Zhao

Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment. The paper seeks to introduce an information theoretic measure as one part of ...

Journal: :Drug Delivery System 2023

Journal: :Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2010
Kazuya Nagano Sunao Imai Shinsaku Nakagawa Shin-ichi Tsunoda Yasuo Tsutsumi

Molecular biomarkers are keys to the development of new diagnostic protocols and therapies. Recently, significant research effort has been devoted to the development of these biomarkers using various approaches. Perhaps the most promising approach is disease proteomics. This method involves analyzing and identifying changes in the expression pattern at the protein level in the diseased conditio...

2017
Matthew P. Padula Iain J. Berry Matthew B. O′Rourke Benjamin B.A. Raymond Jerran Santos Steven P. Djordjevic

Methodologies for the global analysis of proteins in a sample, or proteome analysis, have been available since 1975 when Patrick O'Farrell published the first paper describing two-dimensional gel electrophoresis (2D-PAGE). This technique allowed the resolution of single protein isoforms, or proteoforms, into single 'spots' in a polyacrylamide gel, allowing the quantitation of changes in a prote...

2012
Gertjan Kramer Perry D. Moerland Rienk E. Jeeninga Wytze J. Vlietstra Jeffrey H. Ringrose Carsten Byrman Ben Berkhout Dave Speijer

This mini-review summarizes techniques applied in, and results obtained with, proteomic studies of human immunodeficiency virus type 1 (HIV-1)-T cell interaction. Our group previously reported on the use of two-dimensional differential gel electrophoresis (2D-DIGE) coupled to matrix assisted laser-desorption time of flight peptide mass fingerprint analysis, to study T cell responses upon HIV-1 ...

Journal: :Plant & cell physiology 1996
A K Ekramoddoullah D W Taylor

Recently, a western white pine protein, Pin m III, was shown to be associated with overwintering and frost hardiness of western white pine foliage. To examine whether Pin m III is directly involved in frost hardiness by functioning as an antifreeze protein, work is underway to clone the gene encoding this protein and to assess the function of this gene in freezing tolerance by incorporating the...

Journal: :Analytical and bioanalytical chemistry 2007
Ralf J Braun Norbert Kinkl Monika Beer Marius Ueffing

One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies iso...

Journal: :Journal of proteomics 2011
Masahiro Kamita Yayoi Kimura Yoko Ino Roza M Kamp Bogdan Polevoda Fred Sherman Hisashi Hirano

N(α)-Acetyltransferases (NATs) cause the N(α)-acetylation of the majority of eukaryotic proteins during their translation, although the functions of this modification have been largely unexplored. In yeast (Saccharomyces cerevisiae), four NATs have been identified: NatA, NatB, NatC, and NatD. In this study, the N(α)-acetylation status of ribosomal protein was analyzed using NAT mutants combined...

2005
Reiner Westermeier

Electrophoresis is the migration of charged particles or molecules in an electric field. This occurs when the substances are in aqueous solution. The speed of migration is dependent on the applied electric field strength and the charges of the molecules. Thus, differently charged molecules will form individual zones while they migrate. In order to keep diffusion of the zones to a minimum, elect...

2012
Kazuma Higashisaka Yasuo Yoshioka Kohei Yamashita Yuki Morishita Huiyan Pan Toshinobu Ogura Takashi Nagano Akiyoshi Kunieda Kazuya Nagano Yasuhiro Abe Haruhiko Kamada Shin-ichi Tsunoda Hiromi Nabeshi Tomoaki Yoshikawa Yasuo Tsutsumi

Practical uses of nanomaterials are rapidly spreading to a wide variety of fields. However, potential harmful effects of nanomaterials are raising concerns about their safety. Therefore, it is important that a risk assessment system is developed so that the safety of nanomaterials can be evaluated or predicted. Here, we attempted to identify novel biomarkers of nanomaterial-induced health effec...

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