نتایج جستجو برای: dimensional electrophoresis 2

تعداد نتایج: 2871435  

Journal: :Genetics 2015
Guanjing Hu Jin Koh Mi-Jeong Yoo Sixue Chen Jonathan F Wendel

Allopolyploidization is accompanied by changes in gene expression that are thought to contribute to phenotypic diversification. Here we describe global changes in the single-celled cotton fiber proteome of two natural allopolyploid species (Gossypium hirsutum and G. barbadense) and living models of their diploid parents using two different proteomic approaches. In total, 1323 two-dimensional ge...

Journal: :The Journal of Cell Biology 1985
P Gröbner P Loidl

Purified antibodies from an antiserum against S-phase proteins of the myxomycete Physarum polycephalum were attached to protein-A-Sepharose CL-4B. A late G2-phase extract that contained a mitosis-stimulating protein was applied to this immunoadsorbent, and the mitosis-stimulating protein was enriched by a factor of ten. This protein, which is present in the cell in low amounts, is synthesized i...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1981
C F Aquadro J C Avise

O'Farrell's technique of two-dimensional gel electrophoresis (2-DGE) has previously been applied to the study of intrapopulation genetic variation. This approach assays a larger, and in part nonoverlapping, cohort of protein encoding loci compared to conventional one-dimensional electrophoretic procedures (SGE) and has revealed substantially lower levels of mean heterozygosity. Here we extend t...

2009
By A. Lapin

A two dimensional electrophoretic method is described for the routine clinical analysis of urinary proteins. Cellulose acetate electrophoresis is used for the first dimension, and SDS (sodium dodecyl sulphate) electrophoresis for the second dimension, the latter being performed together with gel staining (Coomassie Blue) on the „Phast System". The Separation media are supplied äs "ready-to-use"...

2012
Fatemeh Tabandeh Parvin Shariati Mahvash Khodabandeh

The term proteomics was coined by Marc R. Wilkins in 1994, whilst he was developing the concept as PhD student in Macquarie University (Wilkins et al., 1996a). Proteomics was mainly derived from the field of two-dimensional gel electrophoresis (2-DE). With the arrival of the concept of proteomics or proteome analysis, the method of 2-DE has gained greater significance. Although alternative meth...

Journal: :Clinical chemistry 1984
O Valiron I Lefkovits P Garderet C Steinberg

Samples prepared from a single batch of labeled cells were subjected to two-dimensional gel electrophoresis and autoradiography. Three factors were varied: total quantity of protein, quantity of labeled protein, and exposure time. The mean background absorbance of the film remained identical (about 0.5 A) for all the treated series, whatever the exposure time and whether or not there were unlab...

Journal: :Genetics 1983
T McLellan G F Ames K Nikaido

Two proteins with known characteristics on one-dimensional gels were studied by two-dimensional electrophoresis to compare the sensitivities of the two methods in detecting genetic variation. Two-dimensional electrophoresis was found to be less sensitive than several types of one-dimensional gels in distinguishing variants of both proteins. Denaturation of proteins in urea in the two-dimensiona...

Journal: :Briefings in functional genomics & proteomics 2002
Lu Charboneau Heather Tory Tina Chen Mary Winters Emanuel F Petricoin Lance A Liotta Cloud P Paweletz

Protein microarrays offer a new means by which to conduct quantitative profiling of disease-associated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of prote...

2006
Mohammad Jahangiri Maria Petrou

Two-dimensional gel electrophoresis (2DGE) is the leading technique to separate individual proteins in biological samples with many biological and pharmaceutical applications, e.g., drug development. The technique results in an image in which the proteins appear as dark spots on a bright background. The analysis of these images is very time consuming and requires a large amount of manual work. ...

Journal: :Biochemical and biophysical research communications 2007
Guihua Shi Tianzi Jiang Wanlin Zhu Bing Liu Huizhi Zhao

Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment. The paper seeks to introduce an information theoretic measure as one part of ...

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