To measure blood flow through fast and slow pathways of the kidney separately, a new method of analyzing dye-dilution curves was devised. Twenty-five patients with hypertension or renal disease were selected for study. Indocyanine green was injected into one renal artery and dye-dilution curves were recorded in blood from the ipsilateral renal vein, using a densitometer. Recirculation effect wa...

Eight cattle (288 ± 22 kg) were treated with 2.2 mg/kg of body weight of flunixin free acid in a crossover design by subcutaneous (SC) and intravenous (IV) administration. After a minimum 1:10 dilution with 50 mM phosphate buffer, a commercial immunoassay was adapted to determine plasma concentrations of flunixin. The limit of detection was 0.42 ng/mL and the working range was 0.76-66.4 ng/mL w...

Time-intensity measurements of ultrasonic-contrast microbubbles based on the dilution theory have been used to assist blood flow estimation. The compartment model has been employed to describe the dilution process. Under the linear and time-invariant assumption, the time-intensity curve measured at the output of a compartment (i.e., blood mixing chamber) is the convolution of the input time-int...

To measure blood flow through fast and slow pathways of the kidney separately, a new method of analyzing dye-dilution curves was devised. Twenty-five patients with hypertension or renal disease were selected for study. Indocyanine green was injected into one renal artery and dye-dilution curves were recorded in blood from the ipsilateral renal vein, using a densitometer. Recirculation effect wa...

We examined Cortisol Kit [TFB] for direct assay of urine cortisol. And the multiplication by dilution factor of urine cortisol values in this kit was examined. The coefficient of correlation of cortisol levels (46 urine samples) between Cortisol Kit [TFB] and Chemilumi ACS-Cortisol II, which is another kit for direct assay of urine cortisol, was r = 0.858, y = 1.86x + 38.2 (p < 0.001). There we...

Antiserum performance in a nephelometric system can be characterized by parameters derived from measuring reaction rates. The characterization process is derived from a series of dose-response curves (elicited nephelometric response vs antigen concentration) generated from various dilutions of the antiserum being tested. Antiserum titer can then be calculated by plotting the antigen concentrati...

ESTIMATION of cardiac output by the indicator-dilution method has been described by Kinsman, Moore, and Hamilton1 utilizing dye; however, radioactive methods for determination of cardiac output owe their inception to Prinzmetal and co-workers,2 who recorded radioactivity as a function of time by means of a counter over the heart after an intravenous injection of radioactive Na24. Nylin and Cela...

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of technical replicates performed and the effect of the volume transferred throughout the dilution se...

We have generalized the microscopic polymer reference interaction site model (PRISM) theory to study the structure and phase behavior of polymer-tethered spherical nanoparticles in a dense homopolymer melt. In the absence of a polymer matrix, fluids of such hybrid nanoparticles show strong concentration fluctuations indicative of aggregate formation and/or a tendency for microphase separation a...

BACKGROUND Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle () standard curve quantification, which requires the time- and labor-intensive construction of a standard curve. In theory, the sha...