In this study, it was aimed to investigate the correlations with leptin, ghrelin, irisin and insulin levels in blood serum of cattle subclinical ketosis. For purpose, 10 healthy Holstein ketosis obtained from farms Burdur region were used. A diagnosis made according Rothera test performed milk, by performing a general clinical examination animals. Blood samples taken vena jugularis into tubes w...

Two recently commercialized enzyme-linked immunosorbent assay kits, the SRSV(II)-AD (Denka Seiken Co. Ltd., Tokyo, Japan) and IDEIA NLV (DakoCytomation Ltd., Ely, United Kingdom) kits, that detect human norovirus (HuNV) antigens in stool samples were evaluated to assess whether they could be used instead of reverse transcription-PCR (RT-PCR) for routine diagnosis. The sensitivities and specific...

Playing with physical construction kits (Legos, Erector sets, Fischer-Technik, etc.) is a good way to build physical intuitions and spark interest in technical fields. Construction kits for virtual artifacts could have similar benefits, with complementary advantages and disadvantages. Yet we currently do not have construction kits for virtual analogs of physical artifacts that provide the same ...

Playing with physical construction kits (Legos, Erector sets, Fischer-Technik, etc.) is a good way to build physical intuitions and spark interest in technical fields. Construction kits for virtual artifacts could have similar benefits, with complementary advantages and disadvantages. Yet we currently do not have construction kits for virtual analogs of physical artifacts that provide the same ...

BACKGROUND This study compared the performance of five commercially available kits in extracting total RNA from small eukaryotic tissue samples (<15 mg). Total RNA was isolated from fathead minnow (Pimephales promelas) tissues (spleen, blood, kidney, embryo, and larvae) using the Qiagen RNeasy® Plus Mini, Qiagen RNeasy® Plus Universal, Promega Maxwell® 16 LEV simplyRNA, Ambion MagMAX™-96 and Pr...

Hand, foot and mouth disease (HFMD) is a common childhood infection caused by many enteroviruses, including enterovirus A71 (EV-A71). As EV-A71 is associated with severe neurological disease, early diagnosis is critical for clinical and public health management. In developing countries such as Malaysia, laboratory capacity to carry out EV-A71 IgM detection is greater than that of the gold stand...

To compare the real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays used for the diagnosis of Porcine reproductive and respiratory syndrome virus (PRRSV), a Europe-wide interlaboratory ring trial was conducted. A variety of PRRSV strains including North American (NA) and European (EU) genotype isolates were analyzed by the participants. Great differences regar...

Rapid, accurate and affordable methods are needed for the diagnosis of malaria. Reported here is an evaluation of a new immunochromatographic strip, the PATH Falciparum Malaria IC Strip, which is impregnated with an immobilized IgM monoclonal antibody that binds to the HRP-II antigen of Plasmodium falciparum. In contrast to other commercially available kits marketed for the rapid diagnosis of f...